Gel electrophoresis | Chemical processes | MCAT | Khan Academy

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Learn how gel electrophoresis separates DNA and protein fragments based on size and why one would use agarose gel electrophoresis versus SDS-PAGE. By Angela Guerrero.. Created by Angela Guerrero.
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16 сен 2013

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Комментарии : 179

@liamdo151 6 лет назад

I'm doing this stuff in gen biol lab and I have no idea what my professor is lecturing about ? After watching this 7 min video, I understand the concept completely. Great job

@ricky4680 7 лет назад

omg this is sooo simple and easy.....i really hate my class teacher

@prakharvyas6653 7 лет назад

@ruhidyusifov2372 6 лет назад

yeah same

@cynthiasvlogs6255 5 лет назад

Me too

@bahahos7426 4 года назад

Me toooo :D

@gratefulgiigggs9834 4 года назад

Lol! You just said what I wanted to.

@leezhengyangbrandon5080 10 лет назад

What happened to your voice khan?

@weihaihe9839 6 лет назад

and ur handwriting...XD....its elegant........

@emeltea33 5 лет назад

Yeah, his voice is fried...

@jervon3739 4 года назад

I'm the 200th like

@ludwigvanbeethoven6853 4 года назад

@@jervon3739 🏆🏅

@helens5946 4 года назад

I'm the 204th like. 😜

@emmanueladetunji7529 10 месяцев назад

Your voice made me come back to this video for the third time😁

@aiki8812 9 лет назад

this is just great. It helped me a lot to learn this stuff. thanx

@MissAristen 8 лет назад

Her voice reminds me of "this one time, at band camp"...

@theghostofspookwagen4715 5 лет назад

say my name bitch

@yooperlite 3 года назад

Wrong.

@mimeass 2 года назад

Yea thats true

@jacyblack8004 10 лет назад

Thank you for the great visual. This seems so much clearer now!

@maddielipscomb4666 7 лет назад

Thank you so much for making this video, it was very helpful!

@zoobihan 8 лет назад

This video is really good. I've been struggling to understand the purpose of electrophoresis for my exam. It's actually a really interesting technique when it's described in this video! My textbook and lecture notes are so dull in comparison :)

@yukilino 5 лет назад

Simple, good and easy to understand. Well done. Thanks for the video!

@ApozVideoz 4 года назад

Nice Video! A note is that since as the DNA gets larger, more phosphates group are found on the structure, and thus the electric force is proportionately stronger, what causes different fragments to separate is a gel that allows smaller DNA fragments to pass easily whilst larger fragments much more slowly (similar to size exclusion chromatography where beads are used).

@Kaloras 4 года назад

One of the best explanation videos I have ever seen. Short, well depicted and easy to understand

@asdasd446446 6 лет назад

Thank U guys so much I've really enjoy it and learnt how to do it ..... Perfect and thank U Angela Guerrero.U were so good at it " BIG high 5 "

@HappyDaysIsMe 9 лет назад

This was great! thankyou so much!!

@rishimakhanlal8905 7 лет назад

Your voice is soooooooooooooooo COMFORTING

@gloryogiji5154 2 года назад

The way you broke this down in very simple terms and easy makes me want to cry. Thank you 🥺❤️

@dnaspectrum5540 10 лет назад

This is a great description of one of the earliest methods of separating DNA. It is amazing how far DNA testing has come from the days of gel electrophoresis.

@charleyjr.iriarte7428 9 лет назад

Thank you for the teaching of gel electrophoresis. Bless you.

@makhinasolieva9477 5 лет назад

Her voice is magnificent!!!

@mdkgr 8 лет назад

Hey beautiful tutorial. How did you create the video? Love the drawings you made!

@zolabee 10 лет назад

Such a good video, made things really clear and fun to learn! Nice voice to listen to too - thanks so much! :D

@4Getf00l 4 года назад

Thank you so much. I have no background on molecular biology or genetics but this made it easy to understand.

@reezis1619 7 лет назад

Amazing explanation, thank you.

@mliskicks 10 лет назад

Great vid! Very clear

@furgo1 7 лет назад

Thank you to the lady that took her time to explain this concept , please ignore Ignorance from some comments, again Thank you, well done.

@MannansElearning2021 2 года назад

ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-Fv-vayq5MCA.html (Watch this video for precautions)

@alleycat4409 3 года назад

Omg I love your voice!! And your handwriting, so so pretty. I hope you decide to make more videos ❤❤

@lunallena5519 3 года назад

Muchas gracias por su explicacion.👍

@safeyyasofy5515 9 лет назад

Well done girl 👏👏👏👏👏! You are the best , big thanks 🙋🙋😙✋

@anienyeneokonna1038 Год назад

Nice lecture, please I want to know the parts of the electrophoresis and their uses.

@sohilausamarabie9631 5 лет назад

Very well explained thank u so much

@hananashraf1002 6 лет назад

جميل جدا وفهمت بالشرح ده اوووي شكرا جدا 😊

@jonathanmoron1917 9 лет назад

great video. thanks

@asdasd446446 6 лет назад

Ure like a superstar thank U so much for this illustration "Big heart Emoji"

@Sameen.Rahman 7 лет назад

A very useful video!

@kimsunsuhan1524 7 лет назад

Some of these comments are so rude...ya all ain't grateful enough this video just simplified so much

@krichard2346 6 лет назад

Brilliant explanation!

@sanjoysaha7679 7 лет назад

great work thanks

@sunilKumar-fb8xi 7 лет назад

Perfect.. I mean that deserves one

@ForeignerD 2 года назад

Enlightening , ty

@22rnd 9 лет назад

AMAZING!!!!!!!!!!

@Diagreen19 10 лет назад

explained very well. thank u

@brianahanrahan 8 лет назад

The polarity stated in the video is a little confusing since we tend to think of cations being associated with cathodes, and thus it would be (+) positive. As someone commented before: The cathode is where reduction takes place. The anode is where oxidation takes place. However, the polarity of the cathode, or anode, can be + or - depending on the type of device. In a galvanic cell the cathode would be (+) positive. In an electrolytic cell the cathode would be (-) negative. if your prior reference had you thinking that the cathode is (+) positive, you aren't necessarily wrong.

@Craznman 8 лет назад

+Brian Hanrahan I had to bust out my chemistry book because people were saying that she has the charges wrong, but she indeed has them correct.

@allen7615 8 лет назад

Cathodes attracts cations which are positive charge. Just think of this: what charge would you think attracts positive cations? Negative. So cathodes are negatively charged... For isoelectric focusing, cathodes are usually basic because of negatively charged OH groups which again important for attracting cations. And anodes on the other hand are just the opposite.

@faalsadat1 8 лет назад

Very informative video !

@GitasFoodDiary 2 года назад

woww...it looks so amazing dear. Thank you so much for sharing. Stay safe and good luck :)

@waqarkhan7870 8 лет назад

Thanks a lot!!!

@anythinggoes4588 7 лет назад

Mi English no good, nor nooor, but mi understand this very well mi friend :) ........seriously, appreciated! lol

@rafaelcalderon3681 3 года назад

Great video!

@minkailusanu5069 7 лет назад

Thank You.

@seeknlearneasylectures3704 4 года назад

hmm. very informative 👍

@mayikken8534 5 лет назад

Simple and understandable

@rayz1408 7 лет назад

Thank you so much

@halafr5861 3 года назад

Thank you 🙏

@MisterTutor2010 2 месяца назад

Been doing SDS-PAGE for 22 years.

@saharosamamaray7846 4 года назад

Thank you

@kavinrajakaruna7035 8 лет назад

everyone on a a PC or laptop LISTEN Make the video Full Screen On your Numpad on the Right of your keyboard spam either 9 or 3 Make some SICK BEATZ

@jdavida97 7 лет назад

2 and 9 as well as 2 and 3..... I should be studying X(

@shaki6500 5 лет назад

Agarose GEL also called "Native Gel Electrophoresis" cause this was the first method, they usually call it NATIVE

@johntindell9591 6 лет назад

Thank youu

@annalilyanna2196 9 лет назад

thank you . I like BioM

@arpanbiswas818 6 лет назад

The video was useful

@ebtihalahmed2289 8 лет назад

You are amazing

@israakareem5304 7 лет назад

Thanks

@renski1792 8 лет назад

I love loading samples onto gels

@hanymohany8793 6 лет назад

very nice

@chroniclerofthe70s 9 лет назад

She forgot to mention the use of EDTA and a UV light source to visualize the nucleic acid bands. The protein's disulfide bonds must be methylated in order to keep the proteins linearized.

@elleszabo8655 9 лет назад

Or maybe she actively chose not to include that topic. Hmmmm.

@vickyoli 5 лет назад

First time I see a capital "G" cursive like that in my life...

@gratefulgiigggs9834 4 года назад

It's probably reaching heaven.

@TheDandyLion 10 лет назад

She sounds like Dora the Explorer LOL

@miltoncabrera4073 6 лет назад

swiper, no swiping! swiper, no swiping! swiper, no swiping!

@muxaffarally9042 6 лет назад

Not at all

@lana_del_rei 9 лет назад

If you're doing Western Blotting, do you measure from the top or the middle of the bands (or the bottom)? For example, I have a thick band and am comparing it against molecular weight markers, but not sure where on the band to measure from.

@chroniclerofthe70s 9 лет назад

I suggest measuring the bands from the center of the band which represents the greatest concentration of nucleic acid fragments. However, you may want to consult your advisor or laboratory instructor, or email the company which sells the nucleic acid size ladder and ask them about how their ladder is to be used with the particular nucleic acids you are working with.

@byrongaspard9531 8 лет назад

I don't know why you didn't discuss restriction enzymes and their function in gel electrophoresis. Very important part that was just left out...

@os3720 7 лет назад

I agree

@HarrisonJay 5 лет назад

Shukran(thanks), you just save my 10mks from a test quiz.

@vinaykumardaivajna2470 6 лет назад

There is both increase in mass and charge how they get separated as the ratio of charge per mass is constant plz explain.

@Jamiejaaumei0e 8 лет назад

PAGE is for small DNA & Protein But SDS-PAGE, if I am correct only for protein

@surfmaster9612 10 лет назад

nice handwriting :)

@ArtistryofDebauchery 9 лет назад

This hardly explained anything. Left out entirely the mechanism of action. No mention on the charge differentiation involved in electrophoresis.

@leoncraftmc 5 лет назад

Umm... I'm using the Amgen lab and my "R+" band is over 10k basepairs when I compare it to the DNA ladder. Idk what to write, so I just wrote >10k bp to avoid incorrectness cuz I can't like predict whatever is over 10k.

@nabihachaudhary3462 8 лет назад

its quinn from zoey 101

@temporaryrelief2981 8 лет назад

Lol your right hahaha

@davidluozhang5251 7 лет назад

what's on his right?

@Imubozuu 7 лет назад

Lmao trueee

@bonbonpony 9 лет назад

Soo where to get that agarose or the other substance?

@shrutirathore6000 6 лет назад

small dna fragments move faster because of the voids in agrose gel are presents .. so the small dna fragments can passs the voids faster than bigger takes time to travees through voidss

@medicalminutia 5 месяцев назад

Since you can do DNA or proteins on SDS-PAGE, does this mean that proteins are somehow smaller than DNA since agarose can only do DNA? I always thought DNA was < proteins but guess not

@ToneWV 9 лет назад

I'm suprised that noone caught the anode and cathode charges.

@chifley123 6 лет назад

Anthony Verdun Jr This is 3 years old but I’m writing this for modern viewers.. Anyways she god the charges correct, just the colors were switched.

@gemechubekele2546 8 лет назад

Agarose might be used for DNA fragments of 50 bps- 500 bps, but in reality it is used for for those which are longer than 500 bps while polyacrylamide is used for those less than 500 bps. and is SDS used for DNA? It is not specifically mentioned in the video, but it seems it is implied. It is not necessary to negatively charge DNA( as it is already negatively charged) and that is what SDS does. Even if it is necessary to denature the DNA, Urea is used.

@minhtuando1768 4 года назад

Better than the guy who kept stumbling on words ummm ahhhh uhhh ! Very informative video.

@holypeachy 5 лет назад

Wow someone writing in cursive, I thought i was alone.

@bagucode445 9 лет назад

Can agar be used instead?

@musicbank9255 3 года назад

How do u cut it out of the gel

@duke1013 5 лет назад

Yeheueee

@abdrizack-muaadsheikhahmed7225 10 лет назад

thankzzzzzzzzzzzzzzzzzzzzz

@philippealexandra468 Год назад

Who the fuck hired Vincent Adultman to voice this informational MCAT prep video?

@nearsan2477 6 лет назад

it made it even more difficult

@mehwishhameed2988 6 лет назад

I have a question "Will the DNA get absorb so that it may shown or it will show automatically"

@esa2236 7 лет назад

Shouldn't it be the other way around for the electricity conduction at the beginning of the video? The anode is the reduced conductor which means a negative charge, while the ca+hode is the oxidized conductor that results in a positive charge.

@amyss 5 лет назад

Actually she's right

@laaquino7846 5 лет назад

cellulose acetate can also be a medium

@alexsaini10 10 лет назад

Yes i agree, she sounds like dora.......lol

@DamperPedal 9 лет назад

i thought cathodes were positive and anodes were negative?

@siryogi1563 5 лет назад

sal's voice is better

@RBEmpathy 8 лет назад

*based on their size and electrical charge

@duke1013 5 лет назад

Hey guys

@jhyland87 5 лет назад

Kinda reminds me of chromotography

@mhmdagd2684 6 лет назад

I watched this 3 times still cant figure out what those 3 samples represent? I mean are they the same only mixed with different dyes? Or they are 3 different samples?