Gene knock Out | Neomycin | G418- Casette I Experimental | PYQ included | CSIR-NET JRF |GATE | DBT

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Gene knockout (KO) is a technique by which the genomic DNA of a cell or a model organism is perturbed, so that the expression of a specific gene is permanently prevented.
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Опубликовано:

3 дек 2021

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Комментарии : 89

@BaaYo 4 месяца назад

To access notes and practice questions, please join our Telegram channel t.me/baayo_official

@sandeepkalita7125 2 года назад

Ma'am, keep up your great work. Best wishes.

@crazycoins8923 2 года назад

Thank u mam Your way of teaching superb And you make tough topics easy magically

@ankitasamal8067 Год назад

Loved your video maam❣️I was searching for this video.

@Maitri24 Год назад

You are excellent superb fabulous fantabulous awesome Teacher a big big big thank You dear mam

@sakeenabatool1002 2 года назад

Literally ma'am, I was just searching on this topic. And I got your notification

@BaaYo 2 года назад

Glad it was helpful!

@kapilgrover7767 Год назад

Best teaching

@manikbrahemi7468 Год назад

Best explanation

@Zama528 Год назад

Home work pura hua mam 😀 Thank you ❤️🙏

@tushargupta8301 2 года назад

Thank you so much Ma'am for the informative video. I had a question regarding transfer of gene as said after electroportion vector gets to the genome and the genes get switch, so how the tk+ gene went in the ectopic and not in ideal cell...

@user-ud3yf7sf8u 3 месяца назад

Well done mam

@nasreenshaik19 2 года назад

Answer will be B ,C ,D respectively , thank you ma'am for this awesome lecture and saving me before exams 🥲

@atulsaini5493 Год назад

Thanku mam for this class

@renukamaurya1966 Год назад

Thank you ma'am ❣️

@maheshswami5226 2 года назад

It was very nice video mam , hamesha ki tarah👌👌👌🙏🙏

@BaaYo 2 года назад

Thank you Mahesh

@Reettu153 2 года назад

Thank You ma'am for these series. I literally afraid of the unit 13. Finally it reduces some.

@BaaYo 2 года назад

You can do it!

@Raj-mr9vg 2 года назад

Bro you are good doctor lol

@krishnakshimisra2406 2 года назад

Very nicely explained. Thanks ma'am

@BaaYo 2 года назад

Thanks you

@mayurransing5205 2 года назад

2 minutes silence for those students who don't find this type of fabulous Explaination😁😁

@user-ud3yf7sf8u 3 месяца назад

Right

@firdousahmad6000 2 года назад

Wonderful explanation mam... Answers of the given sequence of questions will be 2,3 and 4 respectively

@BaaYo 2 года назад

🙂

@de2jx 2 года назад

Thank you maam.

@harini8193 3 месяца назад

Ma'am.. I've a doubt.. in ectopic insertion why the "tk" gene got inserted along with the gene of interest ..

@rituparnakarmakar6723 2 года назад

Nice nice nice, thank you so much🥰

@BaaYo 2 года назад

😍

@chinmayeemohanty9488 2 года назад

6-b,140-c,135-d

@Studentkaksh Год назад

Wow mam Best explanation 👌🏻👌🏻👌🏻❤❤

@hi-bg6uv 2 года назад

6-D, 135-C, 140-B. Thank you mam😍

@BaaYo 2 года назад

try again.. their is some mistake

@a2z440 2 года назад

🙏🙏💕💕

@chemistmuhammadbaqir7249 2 года назад

A. O. A Dear respected Mam

@vaishnavibhardwaj9970 Год назад

Mam I do not understand the last few steps having some confusion Please clear I'm preparing for an exam Confusion is that you said at 20.13 that one gene is delete and 1 is functional i understand about the deleted gene but which gene is functional here I mean we take only 1 gene here than which one is functional getting confused here please clear

@abhishekrana5933 6 месяцев назад

Transgenesis or Geneknock out mai kya distiction h mam ?

@mahnoormehar12 2 года назад

Assalam o alikum! Mam plz crisper Cas9 par b Aik vedio bnaye plz

@amitsahoo82 Год назад

Mam please explain resistance and sensitivity

@senjutinath3931 2 года назад

6.b 104.3 135.2 and 4

@meeramishra8213 2 года назад

Mam G148resistance ke liye kya responsible he????

@triptisingh8137 2 года назад

Thank you ma'am 🙏

@BaaYo 2 года назад

Tripti Please share with your batchmates also

@triptisingh8137 2 года назад

@@BaaYo sure mam

@susmitabose7230 Месяц назад

2 and 4 are correct answers

@khushbubedi1426 6 месяцев назад

Thanku

@BaaYo 6 месяцев назад

🤩

@poojagujjer118 2 года назад

Thanks🌹🌹 mam

@BaaYo 2 года назад

❤️

@chemistmuhammadbaqir7249 2 года назад

Please one vedio on monthly calibration of FTIR Nicole iS5 thermo performance

@GhulamHussain-bw9tj 2 года назад

Pls specify its application. Which product u r analysing in ftir?

@BaaYo 2 года назад

noted

@jyotibagda3990 2 года назад

6-b 134-3 135-4

@hi-bg6uv 2 года назад

6-B ---bcoz cell survive in the presence of G14 & ganciclovir 140-C :- no transition 135 - D :- not crrct bcoz it is non homologous define Thank you for crrecting me mam😍 again video dekha acche se thrn qusn solve kiye..phle jldbaaji krdi thi😋

@BaaYo 2 года назад

Perfect ❤️👏👏👏👏👏

@hi-bg6uv 2 года назад

@@BaaYo 😄

@doraemon6853 2 года назад

Ques: 6_D, 140_C, correct or not? 135 nhi samajh aarha ma'am help me

@akankshasinha5063 2 месяца назад

140 ) 3

@harshvinayak2428 2 года назад

Question1 :- B Question2 :- c Question3 :- D

@diyashideb2731 2 года назад

6B 140C 135-4. These are the answers maam. Are they correct? Thank you so much maam for ur efforts🙏❤️

@BaaYo 2 года назад

@akankshasinha5063 2 месяца назад

6. 2

@nesabfiroz1249 5 месяцев назад

Mam 6. Answer b kaise ho rha please explain

@rachanasinha2113 2 года назад

Ma'am can't understand the last question,why the answer is option D? Please help

@BaaYo 2 года назад

They have asked for incorrect statement. did you notice that?

@rachanasinha2113 2 года назад

@@BaaYo Yes ma'am. I am not able to understand the neo gene insertion site.

@user-ob2tp8pw5p 11 месяцев назад

What's the use of tk gene in vector mam

@BaaYo 11 месяцев назад

It is helping in selection of negative cells. Jin cells me random integration hai vo tk gene express krti hai

@user-ob2tp8pw5p 9 месяцев назад

Thank you mam

@akankshasinha5063 2 месяца назад

135. 4

@naveenshankar4493 2 года назад

Add english subtitles guys...

@rudra7847 Год назад

Maam 140 no question 3 ans kaise hoga

@pinkiyadav853 2 года назад

Ques1 (2) Ques2 (3) Ques3 (4)

@BaaYo 2 года назад

well done

@maheshswami5226 2 года назад

Mam, tk ko outside kyo rakha hua h?

@BaaYo 2 года назад

correct and ectopic insertion ko identify krne me help kreta hai . Agar vo cassette ke beech me hoga to vo bhi neo ke sath switch krega aur cell ke genome me integrate ho jayega aur express hoga. to cell ganciclovir sensitive ho jayegi. Hope its clear

@maheshswami5226 2 года назад

@@BaaYo thank you pyari mam🥰🥰🙏🙏

@ranishaw7545 2 года назад

6- B, 140- C, 135- D. Mam check the answers

@BaaYo 2 года назад

Correct 👏👏

@akhilthota3860 Год назад

@@BaaYo i dint understand why 140 - 3 is the correct answer. Can you please explain mam

@rononoazoro1808 Год назад

@@akhilthota3860 he made a construct that was sensitive to neomycin ( so the neomycin gene will be outside of the homologous terminal region and will be left outside even the recombination is taking place ) and resistant to ganciclovir ( meaning the TK gene will also be outside) hence answer is 3 as both the gene will be outside of the homologous terminal regions