How to Calculate CFU per ml of Bacterial Sample? in 3 Steps || cfu/ml in Microbiology

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7 minute video How to calculate Colony forming Unit per ml of bacterial sample?
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0:00 Introduction
0:15| Correct Equation to Calculate cfu/ml of bacterial sample
0:35 | Example question What is the number of CFU per ml of original stock?
0:48| Step 1: Find out the plate with countable no. of colonies
2:00 |Step 2: Find out the Total Dilution Factor (TDF)
4:48 |Step 3: Substitute the Values in the Cfu/ml Equation
6:38 | Summary of Calculating CFU/ml
Serial Dilution Method Protocol Step Wise Explanation
• Serial Dilution Method...
Dilution and Dilution Factor in Microbiology|| How to Calculate Dilution factor in Serial dilution?
• Dilution and Dilution ...
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30 июн 2024

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Комментарии : 95

@biologyexams4u Год назад

Did this video help? Hitting the 'LIKE' and 'SUBSCRIBE' button helps us too. Take care, Stay blessed Thank you so much @biologyexams4u 🙏

@GraftingAvocados Год назад

I'm a farmer not a trained scientist But I wanted to check my own cfu on the biological products I buy. This video is the best step y step. Thanks a lot for shearing your pedagogical skills

@MiorAkif Год назад

I love how concise and to the point this explanation is. Helped me a bunch!

@edwinkaroney5370 Год назад

It has never been made this easy before. Thank you.

@drnickatnyte2688 Год назад

This is the best crash course I could find. Thank you so much!

@dkdrll_ 2 года назад

thank you so much Sir. this is very helpful in doing the research i'm doing right now

@biologyexams4u 3 года назад

Dear Friends Thank you so much for watching @biologyexams4u Please consider subscribing using the link: bit.ly/3kG2kKf A simplified video explaining to calculate cfu/ml of original stock? Hope this will help Watch, Understand and enjoy Biology :)

@molletibhuvanabindu6751 Год назад

Thank you so much for this video sir. It is really helpful for me about my new medicinal development

@ugochiegbeagu9784 2 года назад

This is a nice video. well explained. Thank you sir for the video.

@imeneema4062 Год назад

so gratful to god that i found your video...now i got it

@aleaoleandra7116 2 года назад

Thank you so much sir, it's so helpfullllll

@AmirShahzad-si1hv 9 месяцев назад

A very awareness explanation by good manners 😊

@MH_2456 Год назад

Thank you so much! I'm taking A-level Biology and you explained it so well :)

@biologyexams4u Год назад

Really happy that the video helped. Thank you so much

@muhammadkaleem4251 Год назад

Nicely explained. Thank you

@user-gr4fh9el3y 6 дней назад

You're a holy life saver😭❤️

@RhiannonReed-xg6vr Месяц назад

Thank you so much. This video was so helpful!

@angelicadin08 9 месяцев назад

thanks. it helps a lot

@perlenoir9420 2 года назад

Thank Uu from morocco ❤️❤️

@gustinfinnegan9000 Год назад

Very helpful, thank you for making this video, i have a question, sir what if we have 2 plate for each dilution (duplo) how to calculate ? Example: 10^-3 10^-4 Petri 1 287 31 Petri 2 292 42 Vol of stock transferred = 0.1 ml Thank you, sir

@debabanighosh4950 Год назад

Your videos are excellent, if possible please make some videos on PCR, Elisa, buffer preparation, sds-page, electrophoresis, endotoxin test and their calculations.

@biologyexams4u Год назад

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@jessekujanen5970 3 месяца назад

Thank you so much. I just couldn't figure this out with the materials provided to me by my professors. It's so hard sometimes to figure out these things out for yourself, when the given material is always in a foreign language and has many terms, which you only are familiar with half at the best circumstances..

@biologyexams4u 3 месяца назад

Glad I could help! Thank you so much for your nice words.

@bilill24 8 месяцев назад

Thank you so much ❤!

@bobi9281 Год назад

Nice explanation

@Matt-or9tq 7 месяцев назад

very helpful thanks

@GojoSensei-px9bx 3 месяца назад

Thank you sir.

@rajadurai283 2 года назад

Good sir.easily understand.thank you sir

@biologyexams4u 2 года назад

All the best

@drsirgay9176 2 года назад

thanks man, great video!

@biologyexams4u 2 года назад

Thank you for your nice words Please consider subscribing and sharing with your friends Thank you so much:)

@P_poo Год назад

Thank you so much 😭🫶

@padpakbboong 2 года назад

Thank you

@cartoonanimationlovers-8922 2 года назад

Thank you so much you explained it very well 1st time I tolerate it ^_^ Please I have a Q, might be silly. now after counting it plate, tube (f) looks has low count, but after calculation it will be 10*8 in compared to tube (e). Does it mean higher count (f)? .

@biologyexams4u 2 года назад

Welcome.. Thank you for the question. We often wont consider the plate with low count or high count as the chance of error is high. Hope I answered the question.

@SinhaSahab1 Месяц назад

Very well explained

@abdallahaden5322 4 месяца назад

Thank you soo much

@adriannarodriguez8805 Год назад

Thank you very much greatly helpful

@biologyexams4u Год назад

You're welcome! Just sharing our Microbiology playlist for your reference. ru-vid.com/group/PLpKNQ2U3np9i5tZ5kNNZ-yAGWAHxvG5j1 Stay blessed. Thank you so much @biologyexams4u

@MarykMakwelele Месяц назад

Life saver ❤

@kagurasama2303 2 года назад

Thank you sir 🤭 now Im ready for my exam!!! 😂🤗

@biologyexams4u 2 года назад

All the best Please subscribe, share and support:)

@rezvanmohammadi9834 Год назад

Thanks a lot 🙏

@biologyexams4u Год назад

Welcome Rezvan...Thank you:)

@fazalmaqsood907 2 года назад

Sir how to calculate aliquot factor in endophytic analysis??

@AdnanAfzaal22 Год назад

Great

@mahendrathalari9878 2 года назад

Nice

@arunahsubramaniam7006 4 месяца назад

hi, If we use raw samples there will be have dilution factor. Also how to count for raw samples transfer into plate 0.1ml.

@savitasoni719 8 месяцев назад

Hello sir Thank My question is Sample of E. Colo it is use one loopful cuture on the plate

@hananesaidani7059 2 года назад

c'est quoi la différence entre ufc/ml et ufc/g ? quand on doit calculer par ml et quand on doit calculer par gramme ?

@AdnanAfzaal22 Год назад

Great presentation. Why we took 0.1 ml and how we can take such minute quantity.

@biologyexams4u Год назад

Small quantity; there wont be overcrowding of bacterial cell. We can take small amount using micropipettes. thank you

@dieudonnenyamaifofe9646 Год назад

You can use calibrate your micropipette at 10 microliters (=0.1 ml)

@berlindaantwi605 Год назад

What if all of them are within range, will you find the CFU/ml of each and then find the average?

@a.s9509 10 месяцев назад

What if I want to have a bacterial solution with 1*10^7 for example. How do I have to prepare it with this 6.3*10^7 solution?

@sabinakhatri7734 Год назад

if you diluted 1ml of a sample with 99ml of sterile water then plated 100ml of the diluted sample on plate count agar and 75 colonies were found. calculate the cfu/ml of the original undiluted sample can you please help with this

@syamilahshaarani 9 месяцев назад

From the 6.3 x 10^7, how to make it 10^6 bacterial suspension?

@sajithkarunaratne8965 2 года назад

How do you get ten into the plus numbers? The calculations gives 10 to the (-) values Right?

@biologyexams4u 2 года назад

Hi Sajith We are using dilution factor not dilution.. Hope this video will help ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-EOsYOfKMgS8.html Thank you so much

@sajithkarunaratne8965 2 года назад

@@biologyexams4u OK. Thank you so much for understanding me. Got it.

@avasava7720 2 месяца назад

alternatively, couldn't you use: no. of colonies/ TDF x Volume of culture in mL? As long as the units are consistent between DF and Volume and multiply those two values and then divide that by the colony value

@almacazares3405 Год назад

Thanks times 10 raised to 10000000

@biologyexams4u Год назад

Thank you so much. really appreciate your creativity. happy to know the video helped. Pinning this comment. take care, stay blessed

@ammardilawar510 Год назад

What is the formula if we get countable colonies in two plates ?

@Qson2321 9 месяцев назад

how can we get 10^7 as final when we had 10^6 after 10^5/0.1?

@tanishakumari2958 5 месяцев назад

First E. Coli sample O.D is what we consider?

@rajakumarangb2912 Год назад

how is the voulme plated i.e 0.1 ml is calculated?

@rekhapandey5363 Год назад

i have a broth culture of a bacteria and i need 10^9 CFU/ml concentration of it to inoculate in a material. Can you help me how i can do that?

@biologyexams4u Год назад

What is your colony count? Did you do serial dilution and plated on an agar plate?

@meditationrelaxingpeaceful5632 2 года назад

Someone give the value of total number of cells 83x 10 power 8. Is it possible.. please clear calculation if possible

@souryajyotidash2096 Месяц назад

Hello Sir, I've a question. How to prepare 4×10⁸cfu/ml ? An earliest response will be immensely valuable.

@UnKnown-sp5ye 2 года назад

How did you get 443, 63, and 3 colonies?

@Dineesh86 2 года назад

Why is vol. of culture plated 0.1 ml and not 1 ml as you use 1 ml from the total 10 ml?

@biologyexams4u 2 года назад

If 1 ml is used there is always a chance of overcrowded plates

@dieudonnenyamaifofe9646 Год назад

@@biologyexams4u then the 63.10^6 CFU should be in 0.1 mL not 1 mL and afterward express it in CFU/ mL

@keepgoing6630 2 года назад

How do we know the number of colonies?

@biologyexams4u 2 года назад

Using bacterial colony counter... Thank you

@sharisalee6347 2 года назад

not understanding how it went from 10 to the 6th power to 10 to the 7th power though can someone explain?

@biologyexams4u 2 года назад

Hi Sharisa Lee Which part? Is it the final calculation? 63X10*6 and 6.3x10*7 both values are same.

@sharisalee6347 2 года назад

yes, you were at 10 *6 but final answer became 10*7 so I'm not sure how final answer became 10*7

@sharisalee6347 2 года назад

is it because once you move the decimal over to the left to get 6.3 instead of 63 , the 10*6 changes to 10*7 ?

@hananesaidani7059 2 года назад

what is the difference between ufc/ml and ufc/g ?

@ahlemahle7491 2 года назад

ML for when we use a liquid sample (eg milk) G for when we use a solid sample (eg a sandwich)

@mandeepnain6162 2 года назад

Dilution will be done by adding media or distill water or buffer

@biologyexams4u 2 года назад

Hi Mandeep Phosphate buffered solution or isotonic saline solution (0.85%)is commonly used as both wont causes osmotic shock..other diluents is Sterile distilled water . Often the selection of diluents depends on the further procedure and also the type of microorganisms ... Like for cell count or for subculturing.. Thank you

@rimadessai6625 2 года назад

Sir why we don't consider minus sign of dilution when doing calculations ?

@biologyexams4u 2 года назад

Really appreciate your question.. Here we are using dilution factor not dilution.. Hope this video on dilution factor will help ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-EOsYOfKMgS8.html Thank you so much 👍