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Hydrophobic interaction chromatography ( HIC) | Application of HIC 

Animated biology With arpan
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Hydrophobic interaction chromatography (HIC) separates molecules based on their hydrophobicity. HIC is a useful separation technique for purifying proteins while maintaining biological activity due to the use of conditions and matrices that operate under less denaturing conditions.

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17 май 2024

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Комментарии : 67   
@qetsiyah1766
@qetsiyah1766 2 месяца назад
The visuals are great! They were so helpful for my understanding. Thank you.
@animatedbiologywitharpan
@animatedbiologywitharpan 2 месяца назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@qamaral-ubiedi5404
@qamaral-ubiedi5404 3 года назад
Do you know how long should my HIC column be to isolate GFP from 1g e.Coli?
@Nick.5.9..175
@Nick.5.9..175 Месяц назад
You are amazing! Thank you so much
@animatedbiologywitharpan
@animatedbiologywitharpan Месяц назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@valentinascatizzi6406
@valentinascatizzi6406 3 года назад
Clear and fluid explanation, thank you . greets
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
please share my channel link with your friends and help me to reach big audience
@nareshbarik5384
@nareshbarik5384 Месяц назад
Thank you ❤
@animatedbiologywitharpan
@animatedbiologywitharpan Месяц назад
could you please help me by sharing my contents with your friends
@vg2358
@vg2358 7 месяцев назад
Great explanation, thank you very much!
@animatedbiologywitharpan
@animatedbiologywitharpan 7 месяцев назад
Please share my channel link with your friends and help me to reach big audiance ❤️
@animatedbiologywitharpan
@animatedbiologywitharpan 7 месяцев назад
Please support our channel using super thanks
@shivaniathani8082
@shivaniathani8082 3 года назад
All the videos on this channel are very very useful. Thank you.
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share my channel link with your friends and help me to reach out big audience
@SS-lr6uk
@SS-lr6uk 3 года назад
Thank you sir it was much needed.
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share among friends and help me to reach big audience
@suvismaya5980
@suvismaya5980 3 года назад
Thank u so much sir I had problem in understanding hw they will use HIC for virus purification...now it's clear......😊😊😊😊
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share my channel link with your friends and help me to reach big audience
@suvismaya5980
@suvismaya5980 3 года назад
@@animatedbiologywitharpan sure😊
@maninderdhillon8351
@maninderdhillon8351 3 года назад
Excellent.. presentation .
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share my channel link with your friends and help me reach out big audience
@maninderdhillon8351
@maninderdhillon8351 3 года назад
Sure.. I will do it.. but it will inevitably reach out to millions one day... Keep the good work going..
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
support from audience is my motivation....hope to bring nice content soon
@JJH2004
@JJH2004 2 месяца назад
So basically the hydrophobic analytes take longer to be detected as they interact more with the hydrophobic column?
@animatedbiologywitharpan
@animatedbiologywitharpan 2 месяца назад
Yes the elution time for hydrophobic molecules are more
@sh7arwt3teer99
@sh7arwt3teer99 2 года назад
please if you can explain to me, why the mobile phases containing a concentration of ammonium sulfate promote the binding of many proteins to HIC columns??
@blueyolker4961
@blueyolker4961 2 года назад
i forgot how it worked but i think it had to do with ammonium (NH4) being a keypart of proteins
@srijandey87
@srijandey87 2 месяца назад
Very well explained ❤
@animatedbiologywitharpan
@animatedbiologywitharpan 2 месяца назад
Could you please help me by sharing my contents with your friends group/ college group. I put huge efforts in making these videos but unfortunately not a lot of people are watching this.
@user-rw6fe3vm5b
@user-rw6fe3vm5b 4 месяца назад
great
@animatedbiologywitharpan
@animatedbiologywitharpan 4 месяца назад
Please share my channel link with your friends and help me to reach big audiance
@davidrosen5137
@davidrosen5137 Год назад
Super helpful.
@animatedbiologywitharpan
@animatedbiologywitharpan Год назад
In order to make high quality content consistently, we need support from you. Please support us by using super thanks option. Super thanks icon is present below the video ( a heart sign with $ in it ) . You can support using paytm/ phone pe/ gPay / paypal. Your small contribution means a lot for us.
@bioline8441
@bioline8441 2 года назад
so good.
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Please share my channel link with your friends
@shrutikachaudhary672
@shrutikachaudhary672 3 года назад
Little bit clarity is needed while speaking rest the video was really nice and explanation through diagrams is good.... Keep it up 👍
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Thanks for the feedback....these constructive criticisms would help to improve the quality in future
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share my channel link with your friends and help me to reach big audience
@rukhsarfatima2283
@rukhsarfatima2283 5 месяцев назад
How can water molecules mask the hydrophobic surfaces???
@himankjain5473
@himankjain5473 3 года назад
Sir if i purchase your course then will i be able to get paid videos of other teachers of iit jam
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Yup my friend you would be able to attain all the lectures from all the educators... do subscribe and use my code AP10
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please subscribe to my other channel ru-vid.com/show-UC4IpyopsGWSjaPACNTZLuqg
@sasukeuhiha1131
@sasukeuhiha1131 2 года назад
I used to have a problem understanding HIC but watching this video has made me realize that I also have a problem understanding English.
@aparnaa4051
@aparnaa4051 3 года назад
Need to increase volume a bit . Thanks for the vdo
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please share among your friends and help me to reach big audience
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please subscribe to my other channel ru-vid.com/show-UC4IpyopsGWSjaPACNTZLuqg
@songohan393
@songohan393 2 года назад
Thannnk you
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Please share my channel link with your friends and help me to reach big audience
@CwdenShorts
@CwdenShorts 2 года назад
Another nice effort!
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Please share my channel link with your friends and help me to reach big audience
@tamanaaswal5316
@tamanaaswal5316 2 года назад
Moj krdi sir mst explanation
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Please share my channel link with your friends and help me to reach big audience
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
Don't forget to check out all my playlist
@richajoshi2578
@richajoshi2578 3 года назад
Nice but plzz make videos on animal and plant biotechnology important topics
@rashmiranjansahoo3975
@rashmiranjansahoo3975 3 года назад
Explanation could have been more better with written texts.
@nanhelalgupta476
@nanhelalgupta476 3 года назад
please does better in which don't mine time limitation..
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Thanks for the suggestion
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Please subscribe to my other channel it's in hindi ru-vid.com/show-UC4IpyopsGWSjaPACNTZLuqg
@_CatalystGuy
@_CatalystGuy 7 месяцев назад
Please speak a little bit loud😢
@animatedbiologywitharpan
@animatedbiologywitharpan 7 месяцев назад
Yes , in this video the audio is not good. May be I should make a new video
@hammadak8
@hammadak8 3 года назад
i think u have problem in your voice... kindly focus on it. and content is good but only voice.
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
Ya my mic was not working properly
@hammadak8
@hammadak8 3 года назад
@@animatedbiologywitharpan its ok bro...keep it up
@kuldeepbiotech4014
@kuldeepbiotech4014 3 года назад
Such a low pitch sound really irritating and such things makes the learning distracted. Audio quality always matters when we try to maintain concentration.
@animatedbiologywitharpan
@animatedbiologywitharpan 3 года назад
I took your comment positively....the sound quality would be improved in future
@worldshootout3298
@worldshootout3298 2 года назад
If possible plz tell hindi 🤒
@animatedbiologywitharpan
@animatedbiologywitharpan 2 года назад
thanks for your suggestion.. I will make a video in hindi as well
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