#proteinpurification #inclusionbodies
This video explains about Inclusion Bodies & Protein Purification
What are Inclusion Bodies?
Inclusion bodies are dense aggregates of misfolded or partially folded proteins that accumulate within bacterial or mammalian cells during overexpression.
Significance of Inclusion Bodies
Contrary to their early reputation as "protein waste," inclusion bodies harbor biologically active protein aggregates.
Factors Influencing Inclusion Body Formation?
It's a symphony of factors - from protein characteristics like hydrophobicity and folding intricacies, to host cell elements such as strain choices and growth conditions. Understanding this symphony is the key to orchestrating better outcomes.
Strategies to Enhance Inclusion Body Formation
This can be achieved by controlling protein expression using regulated promoters, enlisting molecular chaperones and foldases to assist folding, and Fusion with solubility-enhancing tags, such as maltose-binding protein (MBP) or SUMO, can enhance solubility and facilitate downstream purification.
00:00 Introduction
00:13 What are Inclusion Bodies
00:50 Significance of Inclusion Bodies
01:19 Factors Influencing Inclusion Body Formation
01:40 Strategies to Enhance Inclusion Body Formation
02:03 Protein Extraction from Inclusion Bodies
02:38 Refolding Strategies
03:08 Advanced Purification Techniques
03:36 Characterization of Purified Proteins
04:04 Applications of Purified Proteins
04:30 Conclusion
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2 июл 2024
