TISSUE CULTURE 101 | PART 3 | Making Initiation & Multiplication Media + Sterilizing with Microwave 

Says "Because I'm lazy" as if on que, the smoke detector with a dead battery chirps XD

I finished my masters in this field and used medicinal plant, I can say its easy for me but the only thing you need just make everything clean 🌷🌷

You are so awesome! I love how you talk about this like it's all easy and you don't have a degree so anyone could do it, but it's really quite a bit of science. You did all the necessary research, built your own (very well organized) lab, developed good technique, edit good videos and run a business. Fucking epic.

I've watched all your series and it's really helpful! I just want to say thank you for your amazing job! Look forward to see your next videos!

Thanks for the tip on microwaving the media. I thought I needed to get a pressure cooker or autoclave, this saves me time and trouble!

Apart from being incredibly informative (thank you so much for that), this actually looks fun! You look like you really enjoy what you do!

The videos have been very helpful. I started my first batch today. I bought florida beauties from Alice. Two problems I had. One, separating all the plants. Some were difficult to determine where to separate. Second, the medium did not get solid, so I then added agar. I bought the MS with gellum. I thought maybe I didn't wait long enough. I keep referring back to your videos. Thank you!

Subscribed because your working on a beer pong table haha great info though

Another great video. Enjoying the series.

Thx so much for these videos- you explain everything so clearly and make it look simple - fantastic content,

Thanks again ! I really appreciate your videos ! Waiting for the next ones ! :-)

Thanks for sharing! I'm about to start as well.

Thank you so much for your videos. They are so informative. I'm in Canada and Im So excited to get started. I'll be receiving my stuff to make the media today from phytotech and I'm stoked to give this a try. I would really love to see some more videos, I have so many questions 😆.

Heyyo thanks for making these videos!

Thank you for the great tutorial 😍😍😍

Hi, Thanks for sharing. For me, the most difficult part will be to sit and work on the floor which I can't do and was too hard to do when I was young. For info: when the containers are hot and if you open immediately the microwave, the containers will collapse. And due to a big difference in temperature, more fresh air with bacteria will enter inside and you will get more contaminations. That's why it's better to wait for the containers to cool. I use a pressure cooker and I have less contaminations when I leave the flasks cool before opening and removing my flasks. Daniel Jheelan from France

Thanks for sharing! Busy figuring out everything I need for my first setup at the moment. These have helped a ton!

I built a tiny 10"x10" flow hood. Just enough space to pour plates and cut up explants.

@Tatum Sweet do you ever sell the media you make? I want to try to put one of my TC babies in the media to try to have them multiply. I heard you still your plants so you have an etsy store or do you do pop-ups.

Hi Tatum! I enjoy your videos! Please, can you name the brand of the media you are using? I can’t find it on amazon thank you

You're awesome, thanks! what are the concentrations of the hormone solutions you are using? I'd like to create a solution from hormone powder

Would you use the same media for plant tissue or is this specific to plantlets?

Hello after preparing the medium if i cut a piece of plant and drop it in the medium will it grow?

You're awesome thank you😭

Excellent Video, very inspiring. I have a few questions: When you order the MS media, NAA, and stuff, Are they delivered to your doorstep? Do they come in special packing? and after you use them, do you keep them in the refrigerator? Or just keep it at room temperature? Should they be used with extra care? Love all your videos. Thanks.

I'm really enjoying the videos. Wondering if you use a separate rooting media after the multiplication media?

Where did you buy the rack for your plants? I'm looking to buy the same one.

Hi. When the medium is cooled, the lid is wet with droplets of water. Is that ok to put the lid on as wet as it is?

Youre amazing thanks

Where do you buy the actual media and chemicals from? None of that is listed

Hi For making my initiation media do we need BA and NAA? Hi

Can someone please answer , what do I need for the first media to make it grow , m&s , bap , naa , ? I saw also ppm? Please 🙏

Please answer me Where do u preserve the hormone stock solution ? Do u use IAA hormone?

I'm looking for this all in MS+vitamins+agar, but can't find it. You have an idea where to find it? or what's it called so i can search for it? thx for the video!!

excuse me what kind of plastic container for heat resistant ?

What is Ba and NAA? Not listed in your Amazaon Lab

Do you use this initiation media for philodendrons?

You are great 👍 , if you have time please make a list of the things we need to acquire especially for total beginner like me. Please Tatum

Did you ever get that smoke alarm battery changed?

How do you know when to move from initiation into multiplication

Can I ask how you know to microwave it for 7 minutes? My microwave is 1000watts and I can’t find any info online..

Get a ring light homie. Thx 4 vids

whats the concentation of BA and NAA 1mg/ml???

Can this recipe be used for medicinal plant?

What is the temperature for doing this

Pytotech have so many premixed, I am bit confused on there websites can you clearly mention the name of the ms medium

Would you be able to help me with any info about the media for figs?

Hello! Where do I find the pre-dissolved BA and NAA stuff you used?

I need too take a lesson can you tell me where

Hi, thanks for video, Im a beginner so i have a lots of questions😊 - so the process you did here is for multiplication process not rooting process is that right? How is the rooting process done then? - can you store the media if you end up making more than you needed? Thanks

Yea girl

Hi there! Thank you for uploading such helpful content!! I just started doing research and wanne know: For each stage of growth is there a different type of media used? That said initiation media is for the first stage and only needs ms?

Hi , Where do you store your prepared media ? How long can you store it for

Can someone tell me what the name of the Tissue Culture group on Facebook?

When do you wrap the lids with plastic wrap? After you add the media or after adding the plants

What actually is all the stuff you are using

Got to @2:40 can't do it. Change tour smoke alarm.

You don't worry about the microwave destroying the cytokines?

Could you share the Philo protocol?

Do you sterilize your deli cups before you use them?

Thanks for sharing yet again!! by any chance are you a zodiac sign Cancer? Thats what I am sensing haha

just an idea, maybe remove entire carousel plate with all 10 containera at once. im gonna try it.

Change the battery in your smoke detector..

The videos are great but your eyes are better😛

hmmmn making tissue media on a carpeted floor sounds like a recipe for disaster

wearing gloves while wearing swimsuit. Lulz.

Very amateurish.