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Preparation of MS Media for plant tissue culture (Demonstration video) 

BioMed GP
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Composition of MS media file: imgur.com/I32r1F9
Note: The media after autoclaving looks yellow in this video. For demonstration purposes, we used a different media afterwards to save time, however, please keep in mind that MS media does not change color like this while you do this experiment in real.

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16 янв 2022

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Комментарии : 32   
@pushpalatha9867
@pushpalatha9867 11 месяцев назад
Excellent work
@prathapagiriharish2687
@prathapagiriharish2687 Год назад
Nice explanation man
@shantiagrotech
@shantiagrotech 7 месяцев назад
very good video for Beginners. Medam where can I purchase the MS MEDIA.
@jesussaves3904
@jesussaves3904 8 месяцев назад
clear explananation thank u
@harshbhosale4344
@harshbhosale4344 Год назад
What is stock A %
@meetasrivastava3852
@meetasrivastava3852 Год назад
What is the amount of sucrose to be added if it is absent in MS powder ?
@babusona3217
@babusona3217 3 месяца назад
30 gm
@mohammadasadullah5171
@mohammadasadullah5171 2 года назад
If I dissolve all the materials into water and adjust ph than also add agar. But I have no time to autoclave. how many days can keep the media in 4 degree? Or I need to prepare again
@Floral39
@Floral39 Год назад
It should be fine at 4C for 1 or 2 days
@avisiktachakraborty3438
@avisiktachakraborty3438 Год назад
Chemical biological products like waterpowder
@x1Conan3Spartan0x
@x1Conan3Spartan0x Год назад
I was confused on the recipe until I seen this video. Does the Pre-made MS powder have rooting hormone?
@steamerpowered
@steamerpowered Год назад
no, you add whatever required hormones for what your are growing.
@yashikanalla299
@yashikanalla299 6 месяцев назад
Can you please make a video on how to prepare mineral salts media liquid and how to prepare stock of micronutrients, also how yo use ph meter acid base and calibration.. Thank you
@biomedgp
@biomedgp 6 месяцев назад
Sure :)
@yashikanalla299
@yashikanalla299 4 месяца назад
Thanks ❤
@kishanmanch1094
@kishanmanch1094 Год назад
Please kaha melega
@neilsontamang3251
@neilsontamang3251 10 месяцев назад
What did u add to lower the pH
@babusona3217
@babusona3217 3 месяца назад
Naoh
@seethamahalakshmi6651
@seethamahalakshmi6651 Год назад
😊
@burjisbhat8633
@burjisbhat8633 Год назад
Autoclave temp for media?
@babusona3217
@babusona3217 3 месяца назад
15 psi pressure
@surenderchilwal1018
@surenderchilwal1018 5 месяцев назад
what is the composition of stock B,C,D
@biomedgp
@biomedgp 5 месяцев назад
I have attached a file in the description of this video. You can find the detailed composition in that. Please do subscribe to my channel. Thank you.
@sambitbanerjee7843
@sambitbanerjee7843 Год назад
How to measure ph
@biomedgp
@biomedgp Год назад
Using a pH meter.
@urwaali3696
@urwaali3696 8 месяцев назад
Did you add Agar at the end when it solidifies? Please reply!
@frienson_
@frienson_ 7 месяцев назад
The media does not solidify until agar is added as it is the solidifying agent
@babusona3217
@babusona3217 3 месяца назад
Or use gellan gum
@prathapagiriharish2687
@prathapagiriharish2687 Год назад
Mam
@izzapz
@izzapz 2 года назад
Why MS medium is brown?
@biomedgp
@biomedgp 2 года назад
Hello. I have mentioned this in the description. The media shown is some other media. This was only a demonstration.
@danieljheelan5256
@danieljheelan5256 Год назад
It depends on the agar you are using. Some looks more brown.
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