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How to Make Tissue Culture Media (Demo)

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Happy to answer any questions in the comments! Making tissue culture media is the easiest part of the micropropagation process because it doesn't need to be done in sterile conditions, since we autoclave it. Be careful with the pressure cooker. In the future, I do plan to make a video specifically about using a pressure cooker as an autoclave.
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Опубликовано:

3 фев 2023

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Комментарии : 57

@scrummyvision Год назад

awesome introduction vid, just learned this was a thing. brings me back to chem lab freshman year of college XD

@36nibs Год назад

Thank you so much this was really informative ‼️

@busuci63 Год назад

This helps me alot, thank you

@maxlevene1280 9 месяцев назад

Awesome video! Do you have plans to create protocols to follow along with these videos?

@muddypawswanderlust Год назад

Cool video!

@elikarkanane9643 Год назад

Awesome, many thanks

@julious3603 Год назад

Great video! Do plants grow in those little plastic containers after a while?

@tommylu2412 4 месяца назад

Thanks the clip is very informative. Got two questions: 1)do Auxins break down under high temperature and 2) at what temperature do you normally set the magnetic stirrer? Massive thanks in advance

@scienceinfo9965 7 месяцев назад

Which plants are feasible for these protocol?

@chomu3275 Месяц назад

Can you plz help me the hormones needed and quantity for callus formation

@agrippanyagwaya2308 11 месяцев назад

Can I use jam or mayonnaise bottles with solid caps

@linzyweber7247 Год назад

Great videos! Is there a recommended temperature for storing the jars of media with cuttings?

@plantsinjars Год назад

The protocol that you follow will specify the optimal temperature! I don't have the AC in my home lab zoned separately from my home, so these plants stay around 72 - 75 degrees, but some protocols will call for a slightly higher or lower temp.

@JanMinarikCz Год назад

How long it takes from zero to 100 percent? I mean to do through all stages of TC to the point of acclimation? Like 30 weeks?

@adricerq 9 месяцев назад

Hello Laur, Thank you for the quality content! I’m hooked! I’m from Brazil, there is zero information in Portuguese abou the subject. How can I get a pdf of the book you mentioned?

@MaRciNS0001987 7 месяцев назад

When you google "plants from test tubes pdf" the first result should be the 3rd edition of the book as pdf.

@geriannroth449 Год назад

Great

@pricklyplant0333 Год назад

Very helpful! You just earn a sub. I have questions. For tc jar, is it necessary to have small ventilation hole? Ive seen some use air tight glass jar. Which one better? Do tc plants even need ventilation?

@plantsinjars Год назад

For the multiplication and rooting stages, you want the jar to be completely sealed with no ventilation hole to maintain 100% humidity. To prepare the plants for the final stage of tissue culture, acclimatization, you may change the lid to a vented lid, to gradually acclimate the plantlets to lower humidity before you take them out of tissue culture and plant them in soil.

@pricklyplant0333 Год назад

@@plantsinjars got it thanks!

@assassins3178 8 месяцев назад

where could i find the protocols for cannabis? thanks.

@todo5603 26 дней назад

Plant hormones are heat labile , wont it degrade during autoclaving?

@davesplan84 Год назад

Thank you for this! Silly question: None of the components added to the media are degraded by the autoclave process?

@JesusGonzalez-gr9wz 11 месяцев назад

Some plant growth regulators are slightly degraded when autoclaved, but it is ok. Antibiotics on the other hand, should be filter sterilized and added after autoclaving.

@davesplan84 11 месяцев назад

Thank you!@@JesusGonzalez-gr9wz

@saint85 9 месяцев назад

Can I use hoosier hills farms agar for tc

@fshah48 8 месяцев назад

Bravo, I like your educational videos. Please make a video about cloning bamboo and burma teakwood.

@kimratanasakkim 3 месяца назад

Can you suggest the full set ingredient for making the medium for cannabis?

@Chrischem912 3 месяца назад

Please can you list the ingredients

@richietipene Год назад

How do you find out what PGR you need for specific plants?

@plantsinjars Год назад

I use research gate and google scholar to find protocols

@damienwilson6610 10 месяцев назад

Has anyone found the protocols for cannabis?

@Austin-qk9gp Год назад

Do the plastic pipette attachment things just go in a normal trash can?

@plantsinjars Год назад

Yes. I have a special container for disposing scalpel blades but the pipettes get thrown in the normal trash

@Plantsforlifelove 2 месяца назад

Could you help me find a media for monstera / philodendron please 🙏

@plantsinjars 2 месяца назад

I share the recipe in my most recent vid :)

@muddypawswanderlust Год назад

What is the brand and model of your PH meter? And how often does it need calibration? Do you store it in KCl solution? Are u satisfied with its accuracy?

@plantsinjars Год назад

It's called VIVOSUN 3-in-1 Digital pH Meter with ATC, ±0.1 pH Accuracy on Amazon. I calibrate it every time I use it. This one doesn't get stored in kcl. I will probably replace it within the year though, they are known to become inaccurate after awhile.

@muddypawswanderlust Год назад

@@plantsinjars do u recommend vivosun ph meter for beginners like me? I'm so confused about which brand I should buy......😵‍💫 Ps: I can't spend more than $50.

@plantsinjars Год назад

@@muddypawswanderlust I would just get a cheap one like this: www.amazon.com/VIVOSUN-0-05ph-Accuracy-Readout-Temperature/dp/B06XKMH86J/?_encoding=UTF8&pd_rd_w=93qkp&content-id=amzn1.sym.bc5f3394-3b4c-4031-8ac0-18107ac75816&pf_rd_p=bc5f3394-3b4c-4031-8ac0-18107ac75816&pf_rd_r=4E96BEPDMG0F0Z0KMR1B&pd_rd_wg=si6PP&pd_rd_r=cfff3a88-e779-4f4f-9dbd-3328e0c64201&ref_=pd_gw_ci_mcx_mr_hp_atf_m

@gusmanagusmana6669 Год назад

So you are using 1:1 auxin and cytokinen? Does it has particular goal with the ratio? Thankyou.

@plantsinjars Год назад

I was just following a stage I african violet protocol that I found in a book. Different plants / stages of tissue culture will have different ratios though

@gusmanagusmana6669 Год назад

@@plantsinjarswow thank you for your rapid reply😆 i mean auxin and cytokinen affect each other right? So if it's in the same ratio what is the aim, is it to grow the root or shoot?

@letsgobrandon8054 8 месяцев назад

Can you make a video or comment a recipe for cannabis.

@KeithKreider Месяц назад

She just showed you

@tamarafox7386 11 месяцев назад

So wonderful!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!!! =)

@peachtreeunited2346 3 месяца назад

@tonywehnert8483 3 месяца назад

Does anyone know if they are available in Germany? Under which name? Couldn’t find thisstuff….

@plantsinjars 3 месяца назад

There are some links to EU suppliers on my discord server! Link to join is in the description of my more recent vids

@user-vd9vy3ff8t 8 месяцев назад

Я правильно понял, что крышки на банки в автоклаве просто накладываются, не закручиваются, а закручиваются только после автоклавирования?

@underwaterdream2870 2 месяца назад

Wish the ingredients were cheap and easily available as well ;)

@HappyDayRoots 11 месяцев назад

❤❤❤❤

@donniebrasco11 Год назад

Shouldn't you wear gloves when handling these hormones ?!

@andrewdarland8600 7 месяцев назад

Usually… yes. But I’m pretty sure she uses kinetin as her cytokinin of choice. While it’s not 10000% harmless it’s naturally derived and a lot less harmful than say BAP etc. I personally don’t even want to be in the same house as BAP.