On 'Individual 1' wouldn't fragment lengths 1 and 3 still be attached to the DNA at that specific loci given the restriction enzyme hasn't cleaved the DNA strand before fragment 1 or after fragment 3? Same goes for the second individual? I thought that VNTRs were cleaved at the flanking region sequence sites before and after the tandom repeating DNA sequences?
sir firstly thank you for the wonderful explanation. I want to ask how I understand what is mean by heterozygous, mutant, and wild type result written in research papers after doing RFLP on PCR basis? please explain
Maryam, Restriction enzymes are very specific and cleave a particular sequence only which are called as Restriction site. Each individual has different number of occurrences of these Restriction sites therefore different lengths of DNA are obtained. Hope this helps.