restriction fragment length polymorphism (RFLP) |Principle | applications | limitations 

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In short Rflp use southern blot method. Watch the video : ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-n2bVFcJP-HY.html

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Thanks for your suggestion, we will incorporate it.

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in RFLP the GOAL is to detect specific DNA fragments after restriction enzyme digestion and gel electrophoresis. Now imagine the fragments are not transfered in the membrabe. Then hybridization is not possible. In gel hybridization cant be done.

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Noting can be done very accurately with this method. Its obsolete because of highthroughput sequencing. Most RFLP markers are co-dominant (both alleles in heterozygous sample will be detected) and highly locus-specific.

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@@animatedbiologywitharpan Ok

Not reliably ....point mutation might change a restriction cleavage site hence the restriction fragments in theory should be of different length in control and mutant. But now a days pcr based methods / sequencing methods are used

Pcr can amplify upto 500bp dna reliably beyond that efficiency drops badly. So in short yes it could be done but for short dna fragments but not the entire genome.

RFLP is a labor-intensive procedure that does not have the resolving power anywhere closer that of WGS and SNP analysis.

If SNP alters a restriction sequence can be genotyped by 'natural PCR' after restriction digestion.

So it's mean we use SNP analysis for RFLP?

Did you know SNP analysis techniques??

Sir reply plz

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