Reverse transcription PCR - RT PCR procedure

Подписаться 2,1 млн

Просмотров 294 тыс.

50% 1

Reverse transcription PCR - This lecture explains about the principle of reverse transcriptase polymerase chain reaction also known as RT PCR. RT-PCR (reverse transcription-polymerase chain reaction) is the most sensitive technique for mRNA detection and quantitation. Watch this video lecture to understand the mechanism behind the reverse transcriptase PCR to amplify target RNA sequence by reverse transcribing it into complementary DNA.
For more information, log on to-
www.shomusbiology.com/
Get Shomu's Biology DVD set here-
www.shomusbiology.com/dvd-store/
Download the study materials here-
shomusbiology.com/bio-material...
Remember Shomu’s Biology is created to spread the knowledge of life science and biology by sharing all this free biology lectures video and animation presented by Suman Bhattacharjee in RU-vid. All these tutorials are brought to you for free. Please subscribe to our channel so that we can grow together. You can check for any of the following services from Shomu’s Biology-
Buy Shomu’s Biology lecture DVD set- www.shomusbiology.com/dvd-store
Shomu’s Biology assignment services - www.shomusbiology.com/assignment -help
Join Online coaching for CSIR NET exam - www.shomusbiology.com/net-coaching
We are social. Find us on different sites here-
Our Website - www.shomusbiology.com
Facebook page- / shomusbiology
Twitter - / shomusbiology
SlideShare- www.slideshare.net/shomusbiology
Google plus- plus.google.com/1136485849827...
LinkedIn - / suman-bhattacharjee-2a...
RU-vid- / thefunsuman
Thank you for watching the video lecture on Reverse transcription PCR .

Опубликовано:

14 фев 2016

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 274

@Gbemi78 4 года назад

Sir, you don't know how much your videos have helped a lot of people. you really don't know. I just appreciate your effort and God bless the womb that gave birth to you. Gracias

@shomusbiologyofficial 4 года назад

Thank you so much for appreciating my efforts. All the best for future career

@nailaperveen4113 4 года назад

@@shomusbiologyofficial KINDLY MAKE A VIDEO ON GENOMIC SELECTION AND kASP MAKERS ....IT WOULD BE A GREAT HELP

@SarojTamang-kv2ne 2 года назад

So true i do agree

@londonspade5896 Год назад

Bro you don't even know how much you helped me, years ago, when I was studying at university, and then more recently when I applied to work in a Covid testing laboratory. Your videos were what helped me get my degree, and my job, thank you so much.

@shomusbiologyofficial Год назад

Glad to hear that. Stay tuned

@reilian Год назад

same i swear to god his videos are life saver

@londonspade5896 Год назад

@@reilian 100% better than what university provided to learn with

@ousaxx 8 лет назад

You have a video on everything! Such a boss, thank you! :D

@nasera9859 5 лет назад

I am thrilled to bits that you are in RU-vid sharing easy to understand biology. Thumbs up buddy!

@shomusbiologyofficial 5 лет назад

Glad to hear that

@yumnafouzer5923 4 года назад

i'm on the verge of literally giving up on bio and then your videos are there. wow! thank you so much

@shomusbiologyofficial 4 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@yumnafouzer5923 4 года назад

@@shomusbiologyofficial gladly doing that!

@vijayag8116 3 года назад

@@shomusbiologyofficial 🙏me ❤️

@daviddelatorre8288 7 лет назад

Hi. I want to sintetize de Oligo dt(12-18) and Random Primers (d6)n and save some money. How do I have to configure de requirement (order) for the primer syntesis lab? I know that the 5' of the oligo dt must be modified wiht a PO3, but that's all I know lol... Please, help me

@ismaqaiser9431 5 лет назад

Superb explanation....Thanks for giving us such a better understanding regarding RT PCR.

@iloveegypt1324 8 лет назад

Excellent ... very beneficial and simple thank you very much for all your unique videos, I appreciate all of them.

@roseb2105 6 лет назад

why cant one just use 37 degree temperature even with multiple repeats when pcr process happens doesnt it act on ( multiple strands and sequences at the same time i dont understand the thing about higher temperatures with repeats?

@minhanadim7571 2 года назад

Is there any video lecture/demonstration available on "Nucleic Acid Sequence-based Amplification"(NASBA) over your channel because I couldn't find it? If you've not, then pls it's a request to make one lecture on it.

@sagarikamaitra9619 3 года назад

Sir I have question regarding an experiment utilising qRT-PCR: what does it mean to normalise a particular gene (in RNA form) into a GAPDH mRNA and further to cell numbers?

@seeramapoorva6792 3 года назад

Doing a Ph.D. related to molecular bio with zero background in Bio, your videos have and are helping me a lot. Can you please make a video on how to analyze sequencing data from NGS.

@shomusbiologyofficial 3 года назад

Glad to hear that you're getting benefit from my lectures

@openwrt5504 7 лет назад

Like an expert. Great.

@professorsanamalik1650 7 лет назад

sir excellent way of explanation.i must acknowledge:)

@vaishalisharma7773 3 года назад

thnk u again sir... cleared all the doubts regarding this topic

@hsal3093 6 лет назад

Is reverse trancriptase pcr the same as cdna library? Which then is used for pcr? Im sooo confused

@judithjara001 8 лет назад

excellent explanation. . super clear ... thank you so much!!

@otgonsurenmunkh-ochir1202 4 года назад

you are really brilliant teacher i’ve ever seen 😇

@elegansist 8 лет назад

Well done! This is well and simply explained.

@danielvizcaino5970 8 лет назад

Thanks! Great job explaining

@dhbtna 7 лет назад

Very good explanation as other videos of yours. I like board explanation more than the animations.

@amicablemisbah6360 6 лет назад

just wow sir!!! I follow you lectures...

@misbahhussain1021 2 месяца назад

U r the legend sir b4 8 years still perfectly u explained it ❤

@shomusbiologyofficial 2 месяца назад

Thank you

@roseb2105 6 лет назад

would we then determine the amount of dna produced by analzying its quantity on a spectrophotometer and then from that deducing the quantitry of rna present

@nabojitkar1147 7 лет назад

one question: how will you know whether a homolog of a known bacterial gene is present in an unknown bacterial genome? confusion between PCR and Souther Blotting

@Abhishek-ou1gc 3 года назад

Came here to understand RT-PCT techniques currently being used in Covid detection ❤️🔥🙏🏼

@shomusbiologyofficial 3 года назад

You're welcome

@user-qi7qm4oy7z 3 года назад

No bro that is Real time PCR which is qRT-PCR used in covid19 detection

@sahewa100 6 лет назад

Really valuable information sir....go on...all the best !

@newboyshostel 3 года назад

Sir, can you make video on Polymerase Spiral Reaction (PSR) or LAMP PCR..? As there is no video on this topic on you tube and I hope I will get on this platform only . Regards.

@mallikanagamanickam5535 5 лет назад

Sir u mentioned tat rtase won't survive at higher temperature Tan 37 rite for linearising of rna we r heating it to 70 so won't tat denature tere itself

@mayahmed8771 4 года назад

If I use two type of primer will the cDNA be one strand or multiple fragments?

@ashishyt7953 2 года назад

Sir I was suffering with a confusion in this reverse transcription all thanks to you I got all my concepts cleared thankyou sir ❤️

@shomusbiologyofficial 2 года назад

You're welcome

@abdulsaif1607 8 лет назад

sir,, can u please post a lesson on Upstream and downstream processing..

@Abdulsalam-ln4pv 7 лет назад

clarity is Awesome

@Jumpingfrog1234 8 лет назад

Great video. Well explained sir.

@sanskardauneria1070 6 лет назад

Sir question**there is uracil in place of thymine as a base pair in RNA but in DNA we have thymine so from where did the thymine come from two RNA that have uracil in reverse transcript ion

@user-mn8gv6up9b 5 лет назад

it was rly helpful. Thank you so much

@krishnabaruah7185 5 лет назад

Grt learning..thnku sir

@tilakchandrakar6895 3 года назад

Thank you for excellent explanation of RT-PCR. You described it well and minutely differentiate the similarities and dissimilarity's between the similar term's.

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@saichandramacherla7661 7 лет назад

explained is outstanding

@nissiravuri3063 Год назад

Excellent teacher for me ...forever sir,thank u so much sir...god bless you and ur future

@shomusbiologyofficial Год назад

Thank you so much for appreciating my efforts

@Student_2324 Год назад

I am in class 11 and we are making a model of rtpcr in sci exibition , What is the function of Cdna ?? Not understood

@meerpeya5139 6 лет назад

Thank you so much.... sir you are awsyme ...the way of ur explanation. ..nice ..your viedo always help me..thanks once again I got 78% from ur viedos

@shomusbiologyofficial 6 лет назад

That's so nice to hear. Congratulations. Please subscribe and share

@shummu1458 6 лет назад

very great explanation sir👍

@bhargavatejasallapalli7648 2 года назад

Can you please add the sources/cite that use?

@ritusharma2434 8 лет назад

Hi Suman, Its very helpful for me but I have a confusion, if we use oligo dT primer, it will bind to poly A tail but in next step you told that cDNA will be used for amplification, it means oligos dT prime will bind to cDNA so how this is possible because cDNA have polyT instead of polyA. I am thinking if only mRNA is used every-time to make new copy of cDNA, because RT enzyme only used to make a copy of cDNA from mRNA.At the end of experiment we supposed to get a single standard cDNA. If cDNA used as template it will make its complementary DNA strand and they will make double strand DNA at normal temperature.And if mRNA is used as template every-time then it will make many copy of cDNA of same sequence instead of complementary sequence.Please try to sort out my confusion.it will be highly appreciated. thank you

@geekymedico.8099 3 года назад

Thank u soo much sir... .. U r lectures help a lot...,🤗🤗🤗🤗🤗🤗🙏🙏🙏🙏🙏

@lebronprasad2632 3 года назад

Can u provide video on mini preparations and purification of plasmid dna

@jacksonwestaway3376 3 года назад

Shomu Bro you are a legend

@Friend_forever_Pure 2 года назад

Sir is RT-PCR , reverse transciptase PCR and qPCR is same or different?

@agustin.8 6 лет назад

Gracias. Muy bueno

@humanoid1960 8 лет назад

mate you are great!!!!

@alabaadewoleadebayo7703 6 лет назад

Thank you more Sir... I do watch and like your videos.

@shreyasuresh9429 4 года назад

Is Rapid Amplification of cDNA Ends (RACE) a type of Reverse Transcriptase PCR?

@pampaniyaarashi6641 8 лет назад

GOOD JOB,,,,,,THANKS

@carolinakohl9543 3 года назад

Thanks for guiding me through medschool!!! You are awesome!!! 😊

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@carolinejilbert2956 3 года назад

What would I do without you.... Thank you so much!

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@nothingggg656 4 месяца назад

Thankyou so much sir for beautiful explanation 😄💫

@shomusbiologyofficial 4 месяца назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@lawinahmedomar1108 3 года назад

thank you so much

@kashifahmad8250 8 лет назад

Dear sir can you post a video to explain RT-LAMP.

@simplescience3959 7 лет назад

Can someone explain the difference between the Random primer and oligodT because he said both of them can bind to any place and start replication.

@shridharhiremath2896 7 лет назад

No the oligo dT primers will bind to the poly A tail ,they are not random.....whereas the random primers bind to the random sequence of the m RNA

@yogaranisivapalan5253 3 года назад

Thank you so much sir. I'm very grateful to you my lifetime.

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@MrDeep5481 2 года назад

Content is good , voice is not loud

@Biologybucks 6 лет назад

Plz make video on fermentation kinetics and how to remember that

@unliuniverse2999 3 года назад

Another nice video from you. Salute 🥰🥰👏👏

@shomusbiologyofficial 3 года назад

Thank you. Glad you liked my lectures

@kashifahmad8250 8 лет назад

you have done a great job brother, simple and easy explained

@shomusbiologyofficial 8 лет назад

+kashif ahmad thank you very much

@johnnyappleseed6887 3 года назад

Are the pcr and rt pcr the same test???

@saminaahmed934 5 лет назад

Thank u so much sir, it is much beneficial nd i do watch ur most of the video, it does help me a lot..!!🤗

@paramjitmahanta8211 3 года назад

Sir what is the time required for the overall process?

@syedaafsananushrat890 2 года назад

thanks a lot. the video is so helpful for me.

@shomusbiologyofficial 2 года назад

You're welcome

@reginadarlong5655 Год назад

May God bless you sir

@shomusbiologyofficial Год назад

Thank you

@antmes5304 2 года назад

Well done ! Clearly explained ! great job

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@katp9116 4 года назад

hey, does the rt enzyme synthesize the complementary strand of dna (cdna to dna) the second time as well? or is an DNA polymerase added to the sample to synthesize the dna strand?

@iqrabatool2739 4 года назад

After cDNA formation RNAase H is added to cleave mRNA and then dNTPs are added for making ds DNA... Reverse transcriptase is not used

@katp9116 4 года назад

Iqra Batool thank you

@amitsharma-tj4nm 3 года назад

🙏 I have a question My question is - which type of RNA sequence present in these viruses like (m-RNA, r-RNA and t-RNA)

@Hanan-wq1op 3 года назад

Thank you for explaining

@shomusbiologyofficial 3 года назад

You're welcome

@gayatrihaldar5570 2 года назад

Sir . You are literally a saviour 💕 may god bless you

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@gayatrihaldar5570 2 года назад

@@shomusbiologyofficial u deserve it all . And that's the least we can do . Even my teacher studied from your vdos. 😘💕

@krishnenasu7110 3 года назад

Thank you its a very nice video

@shomusbiologyofficial 3 года назад

You're welcome

@rohitkumar-et3np 7 лет назад

very nice....

@shaarifaltaf6473 Месяц назад

U r great sir

@shomusbiologyofficial Месяц назад

Thank you

@hayaa.6545 5 лет назад

Thank you, this was super informative

@aboutstudies123 6 лет назад

Thank you very much Sir. It is really helpful👌👌

@shomusbiologyofficial 6 лет назад

Glad you liked it

@rashmisarkar5289 5 лет назад

So nicely explained sir...you are the best teacher forever....

@shomusbiologyofficial 5 лет назад

Thank you. Glad you liked my lectures

@rashmisarkar5289 5 лет назад

@@shomusbiologyofficial most welcome sir...

@poonampandey6008 2 года назад

Sir mrna se primer se jo complenmtary dna vo use hoga cDNA ke liye ??? Plz ans

@shomusbiologyofficial 2 года назад

Yes

@brakstrongarm9551 2 года назад

Another great video. Thanks. I am about to start doing RNA purification and cDNA creation so good to get a theory primer first:)

@shomusbiologyofficial 2 года назад

Thank you

@tainusali4165 5 лет назад

Thanks sir for your tremendous lecture about RT-PCR.

@shomusbiologyofficial 5 лет назад

Thank you. Glad you liked my lectures

@kamalmurtala1368 4 года назад

Kamal from Nigeria, excellent

@pampaniyaarashi6641 8 лет назад

VERY NICE BROTHER ,ONE MEN ARMYYYYY.ALL THE BEST

@shomusbiologyofficial 8 лет назад

+Arashi Pampaniya thank you. Glad you liked my lectures. Stay tuned

@harveenkaur1713 6 лет назад

thank you!!!!

@KK-go9mp 3 года назад

Can RT PCR be positive in case of typhoid?

@aamirhussain. 2 года назад

love u sir.... helps a lot. lov from kashmir 💛

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@shahzadahmad9630 4 года назад

sir your video is very helped a lot of people, but prepeire new more advance and improve

@shomusbiologyofficial 4 года назад

New videos will come soon

@shaghayeghsaeidi7159 Год назад

You are amazing ❤

@shomusbiologyofficial Год назад

Thank you

@sidrajamil8264 6 лет назад

cDNA synthesis and reverse transcriptase pcr (RT-PCR) are same ?

@sarathreghunathan192 6 лет назад

cDNA synthesis is first step in reverse trancriptase PCR

@aliviadalal726 3 года назад

Well explained sir..Thank you so much

@shomusbiologyofficial 3 года назад

Thank you

@jkssbexamupdate 5 лет назад

Sir u r explaining things very well But u r getting more faster Sir if u will teach slowly I think the things will be explained more.Although ur way of teaching is best