RNA isolation by TRIzol reagent 

Thank you for watching. We wish you good results in all your research.

Thank you so much for providing valuable information. ;-) I wish you good luck with your research.

Thank you. Please tell us if you need an English subtitle version of another experimental video. I wish you good results.

Thank you for your question. If the RNA sample is allowed to dry naturally during the final drying step, there is a risk of prolonged exposure, which could degrade the RNA. To mitigate this risk, we dry the sample quickly by applying a temperature of about 55 degrees Celsius. This method reduces RNA damage, such as RNase contamination, and does not affect the RNA quality. We have compared various drying conditions and found that this approach helps us obtain high-quality RNA. Additionally, we continuously use RNase inactivation solution to clean laboratory tables and tools. We also minimize air circulation through air conditioners to prevent RNase contamination from spreading. When necessary, we work within a biosafety cabinet. While our experience may not cover all experimental conditions, we believe you can achieve even better results by tailoring your experiments to your specific laboratory conditions. I wish you the best in all your research.

@@CDRTtube thank you so much for your detailed explain☺️

Thanks for the question. After adding Trizol and chloroform, it is okay to mix vigorously. Vortexing is recommended for some experimental methods. We wish you the best results in all of your research.

It is needed to shake strongly and vigorously. At final you will get three layers (lower phase: organic phase with pink color, interphase phase: DNA phase with white color, upper phase : aqueous phase as transparent containing RNA ).

@@hassandastsooz3389 Thank you for your valuable information. We wish you always have a good results. :-)

Thank you for leaving a comment Next time, when RNA is extracted, a gel picture will be uploaded. We wish you will find good results for all your research.

Thanks for watching. When extracting RNA, precipitate it through 100% isopropanol, and add 75% Ethanol in the next step. This is to remove the salt precipitated along with the RNA during RNA extraction. So, many protocols are configured to contain between 70 and 80% ethanol. We wish you good results from all your research.

Thank you for watching the video. Prices vary by country and currency exchange rate, but we usually get Trizol for around 300-350 US dollars per 100ml.

Thanks for the question. As one of the experimental methods of RNA extraction, our method uses a little heat in the last step so that the extracted RNA does not crystallize and dissolves well. In case there is a problem with the yield due to crystalization during RNA extraction, applying temperature like our method in the last step will help to obtain high-quality RNA. We wish you good results of your research. Thank you.

Thanks for the question. Does the RNase water you asked mean RNase away? RNase away is a commercially available product. When extracting RNA, RNase away is a solution that minimizes degradation of RNA due to contamination of the solution or peripheral equipment with RNase. If you have any further questions, please feel free to ask. We hope that good research results will come from the research you are trying to do.