Still-Air-Box vs. Flowhood | A Brief Synopsis and Real World Test 

That isn't a still air box. That's a glove box. Big difference.

Wait, you didn't let the still air box settle like you're supposed to you just stuck it in with the turbulent air still going. FAIL

You have a fundamental misunderstanding of what a SAB is. The idea isn't to create an air tight seal with gloves, that's just a glove box with turbulent (not still) air currents caused by said gloves. Hence this test is null and void unfortunately. Good comparison of a glovebox and FFU though.

So if you make one easy mod to the SAB your results will be better. Cover the inside of the lid with a cloth that has been wet with a bleach and water solution prior to positioning the box on top. But you still need to give it some time so the particles settle. I have had zero contamination using this little hack. Full disclosure this was not my idea. Sorry to say I forget the name of the creator who put this out but I would like to thank him!

Under 100 dollars is an understatement and 995 dolas for the hepa seems low, does that include the fan box and everything, idk maybe is cheaper in the US.

Can’t read the results on the HEPA filter screenshot! How much was it compared to the still box?

why did you stopped making videos?! you were making high quality information content with high quality sound and video.

so if you just left that machine with the filter on it in the sab would it just filter all of the air in there?

Can someone recommend a HEPA filter for a home built Flowhood?

What kind of sub do you use? CVG? I like your method in your older vids where you would let the spawn colonize the substrate in a bag and then just put the block in a fruiting chamber. To me that looks like a easier, better way over mixing it up and spreading it out in a tub to colonize. You still growing that way???

Question. If i have a tall medical grade HEPA filter ($550) cant i just get some plastic sides cut to form an enclosure and turn that into a "flow hood"... Or heck jsut leave them off whats the difference between a flow hood like you have there and a medical grade HEPA air filter ?

Could we get some new vids if you have the time ?! They’re very helpful

@Fresh Cultivators What if you took and and used a continuous fine mist sprayer with Clorox and mist down the walls so they were damp inside the SAB so particles wouldn't bounce off the walls and be trapped? This is what I'm planning to try so then I can flame sterilized inside the SAB without an explosion. I tried to search for this but I haven't found any information about anybody that tried it.

Great test! Thanks for sharing. Could you please tell me the name of that instrument? The one used to measure contaminants.

I use a bionare air unit with a 99.99 percent hepa on a positive pressure lab and it works fantastic I’ve yet to have a single contam on any of my stuff

bro, the point of the still air box is to wait a while after opening it to let the particles fall you'll have air currents in there with how quick you did it

That’s a glove box, not a SAB. I would be interested in oven tek, and outside open air inoculation to be included

That's not a still air box. It's a glove box, and apparently much worse for mycology than a glove-less SAB, which allows some air to flow in and out. Gloved sealed boxes create turbulence as you move around and the unsterilized air is moving much more than in a non-sealed still air box.

You can get a 24 / 24 flo for about 500 and it can last up to 10 years before a filter change.

good content

how come he didnt test the cheap filters to prove they dont work?

Actual procedure for a SAB is to let it sit for 20 minutes after spray with 70% IPA. You litterally loaded the chamber with the room air by opening the sealed chamber then took a reading? A poor way to come to any scientific conclusion.

Shoule have used ulpa no?

I have 2 issues with this video. That is most definitely not an SAB, and that is most likely not a flow hood (I cant tell because I cannot see it in its entirety). What you are showcasing is what is called a "glove box" as the cavity is sealed, and the gloves are attached. An SAB simply has holes with which you reach your hands through (gloved or not) into the workspace. And, a "flow hood" has an actual hood (walls and topping), or in the case of a vertical hood the FFU sits atop and has three walls creating an enclosure to work within. Either way, there is literally an enclosed workspace (except for the direction that faces you). Now, if I am incorrect, and theres an actual hood attached that I simply cannot see in this video, then my apologies for this particular point. All that being said, theres nothing wrong with either of these tools youve demonstrated in this video. Both will work perfectly fine for mycology with proper sterile technique. My only issue is the incorrect terminology you are teaching to beginners in this hobby.

Wrong instrument and procedure for testing HEPA filter integrity.

🎉🎉🎉🎉🎉

You didn't let the STILL AIR BOX get it's still air tho... You need to wait at elast like 1 hour before starting that timer inorder for the airparticles to actually fall dow on the flooring of the box... This test was sadly not sufficent in that wa, it was cool tho... But not at all accurate.

It just super obnoxious having to work within the confines of a SAB.

this test its not well done, you should at least wait 15 minutes after close the SAB to do the test ...

Attached gloves on a SAB is such a beginner mistake. I hate to see people who don't know what they are doing pretend like they do smh

Spraying disinfectant in a still air box is not optional. It's not just sterilizing surfaces, the aerosol in the air collides with particles and kills any biologicals on them.

While you have a scientific approach, there are many flaws in this testing.

Why overcomplicate things when you can refine them ?