The Promoter Region - Plasmids 101

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Plasmids are so useful because of their flexibility. But how does the plasmid express the gene of interest? For an answer to that, watch our video on the Promoter Region.
blog.addgene.org/plasmids-101...
Our Plasmids 101 videos are designed to educate all levels of scientists and plasmid lovers. For more, blog.addgene.org/topic/plasmi...
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0:00 - Intro
0:33 - Promoter Region Overview
1:48 - Regulatory Element: Enhancer
2:10 - Regulatory Element: Silencer
2:33 - Regulatory Element: Insulator
2:55 - Considerations on Choosing a Promoter
3:55 - Leaky Transcription
4:30 - Synthetic Promoters
4:45 - Outro
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Credits:
Narrated by: Aliyah Weinstein
Written, Directed & Animated by: Quintin Marcelino
Based on Previous Material by: Kendall Morgan
Designs and Illustrations by: Jason Snair
Additional Designs by: Quintin Marcelino
Sound Design & Sound Editing by: Quintin Marcelino.
Music by: Squadda B and The Grand Affair, courtesy of RU-vid Audio Library
Special Thanks to: Jennifer Tsang, Aliyah Weinstein, Brook Pyhtila, Seth Kasowitz, Mike Lacy, Steven DiPrizio, and everyone at Addgene who provided support and feedback on this project

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Опубликовано:

16 июл 2024

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Комментарии : 10

@addgene Год назад

Addgene does not regularly monitor comments posted here, so we may not see your question immediately. We’d be happy to answer any questions sent to help@addgene.org as soon as possible. Please include the name of the video along with any questions so our support team can help. Thanks!

@andrewdecker9489 2 года назад

Wow, this is such an incredible resource. Thank you for putting these videos (and blog posts) together! The animations are endearing, too 😊

@Clover-eh5vh 6 месяцев назад

This is extremely helpful for my research. I appreciate how clear your explanation is!

@user-tr4bx5ij3g 11 месяцев назад

This video helped me a lot , Thank you 👏

@someonewithsomename 2 года назад

This is one of the most interesting and useful video on the subject! I learned so much from it!

@nekto6201 Год назад

Thank you for such an interesting videos about plasmids! I wish there would be some about editing genome with CRISPR-Cas

@dorfinhorg9458 2 года назад

We've heard of the "TATAA" sequences being crucial for RNA transcription. My question is how important is each base of an annotated promoter? Would inserting exogenous DNA into the promoter lead to decreased RNA transcription?

@addgene 2 года назад

Thanks for your question! You are right that the TATAA sequence is critical for RNA transcription. For the rest of the promoter, it is difficult to say precisely how important each base is and it likely varies depending on the specific promoter. Inserting exogenous DNA into the promoter could certainly decrease transcription efficiency, but to what degree would need to be tested experimentally.