What is a LME liquid culture used for in gourmet mushrooms 

1.75 if you're microdosing.

@@YomAlwa3eed lol this is the way

@@YomAlwa3eed it works lol

2.0 speed to cram info

For real 😆

😹😹😹

Subtitles are life changing lol

He does that specifically for you mentally challenged dudes.

I came to the comments section for this comment

Very true, you'd only have to wait a week to see if there are any contams.

Seems to be the standard time to jump to on a lot of Willy’s videos 😂

So 0.5 grams LME per 300 ml’s of H2O = 0.17% same ratio as Mr. Willy. 12 grams is 4% of 300

That comment was longer than his eeexxxppplllaanaatiioon of how much sugar to use. "Don't use more than 4%" boom, there it is. But no I like his videos, I prefer to mute & use subtitles on 1.75x

I've used just 500ml of water and 1g lme. Haven't failed yet

@@alexwalston5469 Thanks for that. Its been a while since i wrote that comment, and i've experimented a lot. For spore germination in LC (which is not generally recommended since spores are inherently dirty) 1-2% LME is about right since it'll enable the mycelium to branch out looking for resources. However, in terms of a healthy LC, let's say it's for for an isolate, one would be looking for 2-4% LME in my experiencce. Thanks for the reply.

I tried several different recipes, some still working, some cultures I had to throw out. All my cultures using 1gram are still good. I don't know the exact reasons but whenever I use more than 1 gram, the culture eventually gets contaminated.

@@armandobenavidez5682 they get contaminated due to having too much sugar. I have found it best to be a little lean rather than excess

I use 5g LME + 0.5g Nutritive yeast + 250ml water for smaller batches (basically my agar recipe) Is there a reason Willy uses such a tiny amount of food ? As for the contam talk, either your culture is clean or it is not. LCs are not for any use other than perfectly clean tissue samples imho, otherwise that sample should be going to agar to continue cleaning up the culture

Love this question. Continue pushing the envelope, sir.

Should be pressure cooking a little longer than that. That may be long enough to sterilize the liquid but not the emdoporses embedded in the vessel. Just saying.

@@somethingforeveryone4206 funny huh ? the guy in the video telling others that he is a "Mycologist" if you watch closely you will see that he is not.

Are there any additives to lc that will help the mycelium more? Apart from the different type of sugar he uses

@@aidanstanley3263 yes, but no use when they are fed by a single simple ingredients.

@@aidanstanley3263 btw: this dude only answers those who pay for the answers in advance.

DME is Dry Malt Extract. that's what us home brewers usually call the stuff. There is also a version that is made with wheat, rye, and there are darker versions to.... LME refers to the color, personally I would prefer if they just called it Pale rather than light. I bet you would be fine using any one of the renditions of DME on the market... I actually plan to test that soon.

@@thorhale Yeah brewing stuff is 100% fine

hey Wes , i saw you said Agar ... so you use the n/yeast in agar too ? i just picked up some to use in agar but now ill try it in l/c . i used to be worried that cause my l/c wasnt clear clear it was nfg , but ive found it does clear up as the culture grows . i thought i was caramelizing it .why is 4 % of 600 ml 24 grams not 1 gram ? do you have teks out there under Wes Tek ? Thanks for any info. you can share . i use a sab but still getting 50 % contambras w/ my agar , l/c is going good . An We Be Chubby ova' he'ah'

@@MostWantedOHA nothing out there for wes tek...just thought since I haven't seen anyone else using yeast nutrient, I would name it. Yes. It will clear, and you don't have to heat it, but it mixes better if you do. Gentamicin will help with contamination if you use it in the beginning..only necessary for wild isolation or if you're cloning a dried specimen that has been touched..I have dipped specimens in alcohol before inoculation and that works too if you can't get gentamicin. There's other antibiotics you can use too, like erythromycin..you don't want to go over 4% sugar...a little over won't kill it. If you were using honey, 24 gr would be correct. If you're using light malt extract, it only takes 1 gram. Idk why, just following the tek. I experimented with the yeast nutrient and found it very successful and any mycelium I have introduced to it thrived faster and looks very healthy..some even floated to the top and started growing, with black spots in the middle that appear to be trying to pin. That was a dry albino specimen I found. Next, I will add y.n. to my agar dishes to see how it works. I expect the same results. It's not necessary, but if it affects the growth in a positive way, then it's a bit better tek. I wanted to put my name on it since I had a successful experiment and no one else has named it that I've seen...just for acknowledgement. If you're having contamination issues in your tubs, add hydrogen peroxide to your misting bottle or tank if you have an auto setup. Also, wear a mask and use gloves. People say it's not necessary, but trust me, if you want your mycelium healthy, contaminate free fruits, and more flushes, then don't breathe on it or touch it. Just like a loaf of bread, it will start mold just from your touch or breath. I have an extract method too that is awesome. I have not released it yet. I'm about to start a few medicinal grows, & I will shoot the method when I have a flush of rieshi or something to demonstrate with.

@@wesstone7571 wow ! Thanks Wes ...i recently tried enthrmicin , they use it in fish tanks it aint cheap either , im good w/ spawning my tubs got 7 flushes from one last year . my contams are coming from just the air in here got central a/c . its only when i open anything to outside o2 that im getting the dreaded tric ...im sure its in here since ive been growing for a year now , gonna step up w/ a laminar flow hood , been shopping for filters an collecting info. i sprayed down some g.t. w/ iso an talk about blu , wow . im having better luck w/ h2o2 doing clones in l/c and agar . the hydrogen peroxide really made a big difference in success rate . you ever check out home mycology , aka mycomadness ...

Thanks for sharing!

Just trying to check measurements how much water do you mix with your .5 gram yeast nutrient and 1gram lite malt extract? Is it a half quart of distilled water? I was going to use this solution to my spawned seed culture once it’s fully colonized then do just a quick mix in the blender then pour that on a layer of rye seeds that will be mixed with my various substrates. Thoughts?

1- DONT WORRY ABOUT THIS TOO MUCH. Not important accept in Summer. As a rule. I immediately putt back LC jar into fridge immediately. Temp shock is not good. Do what you want with the LC Syringe with liquid culture, place back into fridge is better. Fridge temp will not do anything to a Grain Jar. If you can hold the Jar in your hands after boiling, you can give it the needle. Cold or at Room temp. Store a new culture at room temp, once it is healthy, and growing, than place into fridge for long term storage. Watch hot weather and Cold weather too. This affects growth. Get a needle. Lid. Use hot temp silicon and nail.. Tin foil, and Open Jars. Get a couple of jars of boiled honey/distilled water going at the same time. Always have a back up plan. Like using live mushrooms to inoculate GRAIN.

If you have a fresh sample, best thing to do is to clean the outside of the fruit thoroughly, then, using sanitized gloves in your still air box or in front of the flow hood, rip the stem in half longways. The inside of the stem is sterile and that’s the only part you want to use. Be sure that the outside of the fruit doesn’t touch the exposed interior-keep the two halves separated. Then, using a sterile blade, cut out a small piece and drop it onto an agar plate. If the plate grows clean then you can transfer to liquid culture or grains or whatever. If it’s not clean then you can transfer the cleanest little piece from your first plate to a second plate. Keep transferring away from contams until you have a clean plate, then to LC or agar or grains or whatever. You don’t have to worry about isolating a strain because you already started with a clone so you are only worried about dragging along contams which almost certainly reside on the exterior portions of the mushroom. You can transfer the fresh mushroom tissue directly to LC if you are sure it’s clean (i.e. it was grown in vitro) or if you are confident in your sterile technique. Some people use a syringe filled with uninnocculated LC instead of a scalpel or other knife. They use the needle to dig out a tiny fragment of clean mycelium from the interior of the stem, then withdraw the plunger slightly to suck the fragment into the needle, then inject into the LC jar. The fragment might not even be initially visible but in a few days you have a cloud of mycelium growing in there. If you are dealing with a species that has very thin delicate stems then the needle poke tek is pretty much mandatory. In this case you might have to tear the stem in a cross section if it’s too skinny to split longways. You will need a steady hand to snag a chunk of the interior without touching your the exterior of the mushroom with the needle. However you choose to clone a fresh fruit, sterile technique is essential for success because in all likelihood the exterior of the mushroom is contaminated with something.

If you added 24g to 600ml water then the total volume would turn into 624ml. 24 might be 4% of 600, but it won't be if you add it to the 600ml volume. It needs to be 4% of 600. Not add 4% to 600.

@@dsclassic6568 I think this is going over most peoples heads 😂👌🏼

Thanks guys. I thought I was nuts, rewatched the recipe and had to check for this comment. 😅😅 I One man's 4% is not the same as another's. 💖🔬😎💨

Karo actually is a grain sugar so it does not have the recovery time . #MushLove🍄

@@thesmokingrooster8791 what if I’m using LME like in his video, powder form. How would I measure that out to the 600ml total concentration? Could ya help a brotha out with the math? Lol Also, is it still just 2 Cc of spores to each jar?

Does it come out cloudy or murky?

Can you recommend a brand of ELME?

@@glenw3814 just get the kind at the health food store or off Amazon, labeled as extra light malt extract

@@mainegrower 👍 Thanks.

So a couple weeks ago I read your comment and thought oh yeah now I’m not gonna bother with the straining and all of that. I should’ve just listen to the video instead of listening to your comment Bc the issue is that even when you use boiling water and it liquefies after you pressure cook it there’s still going to be a bunch of sediment and your liquid culture is going to look disgusting, it’s also going to be hard to see anything you put in there.

Yes you can, but more chance of contams.. Spores are generally slightly contaminated. About a week I think..

0.22um PTFE

I want to. Do thesame thing can multiple strains be mixed into one liquid culture jar?? What did you find out about this???

Not smart. Keep things simple and labelled. Best to know what you is growing. You most certainly can, but...... you never know what you is growing and the effects of this relationship on LC and fruiting. You want to experiment, go for it, but..... probably a waste of time.

@@chrisregpick Thanks for the advice. Ill probably just go with small jars that only require 1-2cc so these syringes dont go to waste. What are your thoughts fruiting different strains in same chamber?

Same with agar plates after you pour!

patreon probs

Your stupid. 99.9% of us love the info he provided. Your the only idiot who doesn't want it, go read a forum instead ahole

*you're* stupid. You use an apostrophe when the 'you're' is being used in place of 'you are'. I also enjoyed the Info he provided. I just didn't enjoy the 10 minutes of babble before said info. It's quite known that willy knows his shit but is a little heavy winded. Just an opinion. No need to get all huffy

I agree he could have cut that 9 minutes down to like 3 that's the only thing I hate about his channel the long drawn out intros.

I agree. And you werent an asshole about it either. Ur good bro

Have you tried not speaking on behalf of 47.9k people? We don't feel the same. Thank.

Yes it will work. Cook for like 10% longer

@@chewy2420 thanks jeff. I've posted this a few places and you're the only one to answer. I appreciate you friend.

You are both wrong, you are closer though. 600ml of H20 multiplied by 4% is 24ml, which is 2.4 grams of extract.

Those darn decimal points... 1ml water = 1g

Bro, this comment section is some freedom maths.

Over a year and it’s not been corrected 😳 So @thesmokingrooster8791 you were only off by a factor of TEN! In science related matters it’s important to state objective facts… Math is the way, the truth, and the light! • 1 ml of H2O = 1 gram. Fact • 4% in decimal notation is 0.04 or 4/100 Therefore: • 600ml of H2O = 600 grams • 4% of 600 ml’s or grams is written as y=0.04(600) = 24 grams So if the maximum is 4% by weight the range of sucrose would be from 0 - 24 grams per 600 ml’s of H2O. Accuracy is paramount!

Your math is unquestionably WRONG!

I have the math at 24grams as well, He's not even close to 1% but comments are saying worked great?

I made my liquid culture but it always and never miss, it dies back after 3 to 5 days.

I'm wondering if these infos in LC are real or truth.

Is there somebody there give me some working recepee, in a little bit more detail cause i'm just newby, thank you to you. If none still thank you.☺

Walmart or Rural King has them online if you haven’t found one yet.

with water specifically grams and mL are equivalent at 25C, so 1 mL of water is roughly equal to 1g of water.