Absolute legend, massive thanks from a 3rd year struggling on with his Genetics dissertation with only 1.5 years of in person teaching (strikes + a pandemic are a mean combo).
Hi! Thanks a lot for the great lecture series about genome editing! It helps a lot to understand the topic! At 2:38 , do you mean double stranded RNA sequence instead of DNA? Greetings from Berlin!
Glad you found it helpful Christian! Protospacer Adjacent Motif - the protospacer is the first ~20 bases that match the genomic target. The PAM is the next 3 bases in the genomic DNA that follow after the protospacer target, which Cas9 locks on to.
