I have my mock in 2 days and my teacher was so bad with this topic. I've literally been teaching myself half of the whole A level so I hope it goes well. Your videos are a huge help for people like me, and I hope you continue to help as you are. Thank You!
hiya!! your videos are always so helpful to me, thank you for them firstly :) second - i have a question about the third step (synthesis of DNA) - do the 2 strands join together by DNA polymerase or Taq Polymerase? (dna polymerase is whats mentioned in my text book)
Heya i have the ocr textbook- it says DNA polymerase adds bases to the primer, and the enzyme Taq polymerase is used. I'm pretty sure what this means is that Taq polymerase is a type of DNA polymerase, which is used in PCR because it is able to withstand the extremely high temperatures and doesn't denature. Hope this helps :)
Precisely! :) Taq polyermase *is* DNA polymerase found in the thermophile Thermus aquaticus. It is specifically isolated to use in PCR as it can withstand the high temperatures used in the thermocycler.
When the free nucleotides pair up, does the primers remain bound? Or do they leave allowing free nucelotides to bind to the part of DNA it has left exposed?
