Please recheck your explanation of competitive elisa. As the second antibody is of the same type of the first one, so it will not react and no color will be produced. No color = positive result
A sandwich ELISA is more sensitive and robust as the antibody binds to two sites on the antigen. This increases the binding specificity of the primary capture antibody to the antigen as well as the binding specificity of the detection antibody to the antigen.
Video is informative but wanted to bring to your notice that , In competitive ELISA - no Color means positive(I.e no antigen is free to bind the enzyme coated antibody) so the added enz. Coated antibody are washed and on addition of the substrate no Color produced . If Color is produced it means antibody are not present in the pt. Sera I.e on added enz. Coated antibody are bound to antigen on plate and on addition of the substrate Color produced.
What are the reasons to opt for a specific type of ELISA test? The direct ELISA and competitive ELISA seemed very similar, so when do you use which one?
Substrates are 1) Alkaline phosphate - Paranitrophenyl phosphate (blue clr) 2) Horseradish peroxidase - tetramethyl benzedene (yellow clr) The buffer solution is used to wash
Sbko english ka 14 banna h🤦♀️🤦♀️ Bhai atlst hindi m agar sahi se explain kr skte ho to please explain it.. Hindi and english doesn't matter if you have good communication skills.. English just a language please don't do. That
Secondary antibodies provide signal detection and amplification along with extending the utility of an antibody through conjugation to proteins. ... Secondary antibodies help increase sensitivity and signal amplification due to multiple secondary antibodies binding to a primary antibody. I hope this helps