In Situ Hybridization

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This lecture explains about the in situ hybridization process and the role of in situ hybridization techniques in identifying specific DNA sequence.
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Опубликовано:

5 июл 2015

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Комментарии : 249

@manarsmaan2624 2 года назад

This is how the person explain when he loves what he does . thank you so much for the great explanation All Respect

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@wrapupstudio9149 6 лет назад

you are tooo good.... A teacher like you is not even in reputed colleges... thank u so much.. keep going

@renegerritsen6178 3 года назад

This is the third video I watch on this topic and you are the first to actually make it understandable, thank you!

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures. Please subscribe and share

@mireillet4573 2 года назад

This is what you call a good and simple tutorial. Thank you so much !

@shomusbiologyofficial 2 года назад

You're welcome

@xeverydaynormalgirlx 8 лет назад

My favourite part of this video was the part where an ad popped up offering to do my assignments for me. I bet that will help my learning.

@CelestialCookies 4 года назад

God bless you. You make biology as simple as it should be. Thank you very much

@maryamjokar7066 4 года назад

4:10 now that was a perfect circle 😳

@shomusbiologyofficial 4 года назад

Thank you

@umeaiman4168 4 года назад

Sir, u r one of my great teachers...my every problem has solution from ur lectures...

@shomusbiologyofficial 4 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@yimuyang4101 8 лет назад

Thank you Shomu!! Your lecture make it easy to learn, Big help!!!

@rakshithav2271 3 года назад

Thank you so much... trying to prepare for exams. And your videos make them so much easier.

@stephanie_4203 3 года назад

This is awesome. Why I can't find teachers like him¡¡ I understood all¡¡¡ Thank you so much. I will approve my exams now¡¡¡

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@SunandGold 3 года назад

Thank you!! Really helpful, I was so confused by all the websites I used because they over-complicated the terminology and the diagrams! I spent so much time on those websites getting confused, when I could have just watched your video

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@hesnanoussa6442 3 года назад

i do study biology ( biochimestry )but in frrench luckely ur explanation was quite good i have understood quite well

@shikidixit5144 7 лет назад

Thank you, Shomu!

@malikamehmood3281 3 года назад

Tomorrow is my exam, and you know what you are a great teacher and understand each and every thing you've explained, thank you so much ❤️

@mmmmmmmmm4383 5 месяцев назад

Thank you for appreciating his efforts

@aishasoliman8647 8 лет назад

really helped!! thanks a lot I have a question: If I have wild type and mutant models and I wanna examine the expression of specific markers Should I prepare probes for each markers for both mutant and wild type ?

@diegonzalez25 7 лет назад

Muchas Gracias Shomu!!! Saludos desde Paraguay

@alonir101 8 лет назад

Thanks man, you are great and i like your passion about this :)

@sramanagiri3803 6 лет назад

Ur explainations are so worthy to listen

@muskangarana8074 2 года назад

You are really master of microbiology Thanks a lot for the videos 🙏

@shomusbiologyofficial 2 года назад

You're welcome

@coconuttree1972 6 месяцев назад

Sir, You're really something.... ❤🎉🎉🎉

@shomusbiologyofficial 6 месяцев назад

Thank you so much for appreciating my efforts

@RajKumari-ld7ld 5 лет назад

Thanks brother.... U r a brilliant teacher

@chintanmehta4695 4 года назад

Hats off sir! I am speechless that the way you narrate is amazing one.

@shomusbiologyofficial 4 года назад

Thank you. Glad you liked my lectures

@ShahFahad-eo6fm 6 лет назад

It was very helpful bro ,thank you for making the video

@tatyanakiryutina7778 6 лет назад

Thanks! Most helpful information I found so far!

@shomusbiologyofficial 6 лет назад

You're welcome

@AS-mm4pn 5 лет назад

What exactly did you mean by freezing the mRNAs?And how can you separate the 2 strands of a dsDNA?

@nehanasir3492 2 года назад

How is the complementary strand to the target sequence removed before hybridization? Because it may happen that during hybridization or cooling, the target strand again bind to the complementary sequence instead of probe

@RahulKumar-mm5vm Год назад

I have a question regarding dna sequence using FISH. "Is it possible to identify a particular gene sequence (for example - Amel Y) using FISH if there is lot of depurination (loss of adenine , guanine) in interested gene sequence ?"

@kimlynnybaby 8 лет назад

thank you, you were extremely helpful

@jim5400 7 лет назад

Great job Shomu!

@khushshah524 Год назад

I am presenting this technique tomorrow in front of my PI. This guy saved my life. I have a question: what does hybridization mean in HCR? Which step in this process is called hybridization?

@yasirahmad2185 3 года назад

Sir, i m pakistani and loves u sir for ur simplicity and teaching method. InshAllah in future i will come to india specially to meet u sir.

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts. Glad to hear that you're getting benefit from my lectures

@bikramroy3783 4 года назад

Sir can you tell me which book it is present

@prachikamble1004 6 лет назад

Good explanation skills! I like ur all the videos!

@mandisangema2568 3 года назад

how do you know the complementary sequence of the gene i.e. the probe or bait?

@giovannaagnelli6752 5 лет назад

Thank you so much! It was so very hard to find a good video about in situ Hybridization. 🍀🔬📚💘

@cleopatrahiddentalents. 5 лет назад

You r very helpful bro

@saimairshad5074 2 года назад

Sir, is this same as southern and northern blotting techniques?

@nyctophile1841 3 года назад

Amazing way of explaining sir.. Thank you sir for this 🥰

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@chelseagunaratna5249 8 лет назад

Hi Shomu! You are amazing!

@jijizali 2 года назад

NOBODY EXPLAINS ABT FISH BETTER THAN THIS VIDEO OF YOURS SIR THANK YOU SO MUCH

@shomusbiologyofficial 2 года назад

You're welcome. Glad you like it

@moonsevinci88 Год назад

Thank you. Plz how we can separate the strands ? in vivant

@anj_____m 6 лет назад

AMAZING!! BETTER THAN MY SCHOOL TEACHERS!!! THANK YOU!!!!!!!!!

@shomusbiologyofficial 6 лет назад

Thank you. Glad you liked my lectures. Please subscribe and share

@mauliksadhu8963 4 года назад

Is it not same like southern blotting...? What's the difference between these two..?

@priyankatiwari3434 7 лет назад

thanks .....great video as always..

@ushakarreddy1981 3 года назад

excellent sir ..my doubts were cleared by your wonderful lecture..

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@yaseenkhan-im9ti 4 года назад

Aap jaise logon ki wajah se genetics interesting hai

@shomusbiologyofficial 4 года назад

Thank you

@nithyaa5841 3 года назад

Sir what about in situ hybridization using non radiolabeled probes??

@mahyahosseinib.4366 3 года назад

Many thanks, Shomu!!!

@sapirshaba7546 5 лет назад

Hello, can you explain about detecting abnormal chromosomes such as 18,21, by FISH?

@KIRUBAKAR 2 года назад

Bro what's the difference between colony hybridization and insitu hybridization

@DHIRAJKUMAR-xv4sr 4 года назад

Helpfull for me all six lecture related to gene mapping......thanks sir .😊

@shomusbiologyofficial 4 года назад

Glad to hear that you're getting benefit from my lectures

@fikayoadegboyega3580 2 года назад

Nice one. I so much like your teaching skills

@shomusbiologyofficial 2 года назад

Thank you so much for appreciating my efforts

@soumyasudhi3955 5 лет назад

Useful video....Thank u..

@reviewit9100 5 лет назад

Very helpful video for mee😍😍

@pagalchori577 7 лет назад

thank u shomu sir ......

@TIB5 7 лет назад

fantastic video, thank you so much!!!!!keep up the good work. Im subscribing already!

@shomusbiologyofficial 7 лет назад

+-TIB5- thank you. Glad you liked my lectures

@latimerpaul 5 лет назад

Great video, Shomu. Thank you!

@shomusbiologyofficial 5 лет назад

You're welcome

@davidcharles6518 4 года назад

Thanks very helpful👍

@kouapatrick3838 7 лет назад

Thank you Shomu! I would like to know how the 2 strands of the DNA is separated in this process? by heating the cell ?

@ruthzomuansangi9698 6 лет назад

KOUA Patrick i think nick translation is used.

@caterine4604 4 года назад

You are a blessing !

@shomusbiologyofficial 4 года назад

Thank you

@mohsinbukhri9864 8 лет назад

well done bro...

@kissmeimhuman 7 лет назад

fantastic thank you

@neelamdas5565 7 лет назад

soooo nyc bro.... help me a lotttttttt...... yaaaa a lottttttttttttt

@chhoamondal3526 4 года назад

I have understood easily the complex thing from your lecture

@shomusbiologyofficial 4 года назад

You're welcome

@arkadeepmondal4148 3 года назад

Thank you sir

@mansiwadekar1719 Год назад

Thank you Very well taught

@shomusbiologyofficial Год назад

You're welcome

@biologywithbasira 2 года назад

great job! your explanation is amazing

@shomusbiologyofficial 2 года назад

Thank you

@briabriapia 5 лет назад

dam that circle was perfect

@rahishaaz4392 4 года назад

Sir is FISH technique used for serotonin binding receptorss??? Plzz answer

@normadicn5700 4 года назад

Google?

@arias.p4355 3 года назад

Thanks a lot! Your videos are amazing!

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@manisharai9170 5 лет назад

Thanks sir thakq very much

@jyotiyadav9930 6 лет назад

very helpful this vedio for me ✌✌✌

@denvorvalentine1581 6 лет назад

Thank you.

@dasachomova201 3 года назад

Thank you, all your videos are very helpful :)

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@TheHelzaz Год назад

Thank you

@playstug7944 6 лет назад

Want vedio of clia techquie and hb electrophoresis

@seepa3890 3 года назад

It is easy because you explained it in simple way☺️

@shomusbiologyofficial 3 года назад

You're welcome. Glad to hear that you're getting benefit from my lectures

@ramansingh3250 6 лет назад

Thank u bro.

@shivanisehra1487 5 лет назад

Mere exams h next month please ap kuch topics h unpe video bna skte ho plzzzzzzzzzzzzz mujhe smjh nhi aa rha kuch

@hamedhosseini4938 7 лет назад

Thank you Jan.

@prathameshdeshmukh5844 5 лет назад

REALLY HELPFUL....... AWESOME LECTURE.... TY..

@shomusbiologyofficial 5 лет назад

Glad it helped

@sophianathan1637 8 лет назад

thank you!

@unnikrishnank8482 8 лет назад

Awesome !

@emilymarques6739 5 лет назад

Thank you Shomu!

@shomusbiologyofficial 5 лет назад

You're welcome

@totallyomi 4 года назад

Sorry if this is a stupid question but how do we know the DNA sequence we are targetting in order to design the complimentary probe?

@shomusbiologyofficial 4 года назад

Dna sequence must be known prior to the experiment

@user-dh9lg7re1v 4 года назад

اريد كتاب او مصدر بخصوص هذا الموضوع وياريت مترجم بشكل pdf

@mohammadnamarneh2345 3 года назад

You are amaizing 🌷🌷🌷🌷🌷🌷

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@me6d298 4 года назад

Plz add a video on MLPA.

@mayukhroy5393 4 года назад

Wonderful explanation Sir.

@shomusbiologyofficial 4 года назад

You're welcome

@zunairaakram1672 5 лет назад

Thank you shomu...helped me alot,,, cheers from pakistan... :)

@shomusbiologyofficial 5 лет назад

Thank you. Glad you liked my lectures

@dr.bidouranaznin1339 5 лет назад

Thanks! nice & simple way to learn. How much work area is required for FISH setup?

@markj.7687 5 лет назад

FISH set up is basic and does not require much bench top space. a simple table top would work just fine.

@rashmisingh1857 6 лет назад

As i am pursuing PG.....wanna more elaboration of topics.......it wz lil bit helpful

@shwetagupta9004 4 года назад

Thank u so much sir . Aap n hote to pata nahi hum students ka kya hota.

@shomusbiologyofficial 4 года назад

You're welcome

@shalinidas2454 8 лет назад

thank u soo much

@fatimahyassin424 7 лет назад

excellent

@arshiarshi3756 4 года назад

You are right 👍 it is a easiest method.

@supriyaubasupriyauba3217 3 года назад

Thank you so much sir

@shomusbiologyofficial 3 года назад

You're welcome

@abhasingh6959 7 лет назад

how do u arrest cell in metaphase...when done on slide? reagent and concentration please...Nd please elaborate on signals .

@florytalez 7 лет назад

We arrest them using freeze fracturing technique

@NehaSharma-hd2so 3 года назад

U r gem to me and others too.

@shomusbiologyofficial 3 года назад

Thank you so much for appreciating my efforts

@AnkushSharma-zv5hv 6 лет назад

thanx