Innoculating Grain Spawn Jars with Spores || Propagating Oyster Mushrooms PART 4 

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i’d recommend using 70% isopropyl alcohol instead of 91% bc the 91% evaporates too quickly you’ll get it much more sanitary with 70%

Thanks for sharing another great episode in the series of the profargating of oyster mushrooms !! 😁🌱🍄🍄🍄🍄

If you keep your liquid culture in the refrigerator can you add it to the grain while it is still cold? Thank you in advance for your answer. I keep mine in the refrigerator.

This series is A+! Subbed

I enjoy your work very much. Thank you. DOC

Plz guys your needle tip does not need to glow orange when sterilizing just a quick 3-5 once over with the flame then a wipe with the alc is plenty. When it glows orange you can introduce new contams threw flame soot.

i'm here to learn how to make magic mushrooms, thanks for the tip dude.

Very informative, thank you.

What size particulates (micron size) will tyvek filter out? I wonder if its micron rating is small enough that it could be used to build a homade laminar flow filter.

My dumbass forgot the lighter. I also thought the spores went bad but learned thats how these look.

question, what about the filter tape over the hole for the gas to evaporate out ??..i have seen others use the tape .

Please brother what is the injection name

Do you have any more advice on the environment the jar should go in? ie: Light, No light, some light, humid, not humid, really humid. room tempature (Which room) my basement or attic (Sorry, auto correct spelled attack)? So 65 degrees or 90 degrees? Thanks in advance.

hello, will the spore spawn directly into the mushroom substrate instead of grain?

good info thank u

Recommended throwback

I bought pint size for my grains instead. I am guessing 1cc to 2cc? Is this going to mess me up?

After the mycelium fills the jar, how long would the jar last without opening it?

I like your idea, I see guys with rtv injection ports and tyveks or filler fiber. Nice.

so for a half pint jar how many cc do u recommend from a spore syringe

You should put links to the other parts so we find the whole series

What about the other side of the filter, did you sterilize that before closing the jar?

The difference between doing science and just fucking around is writing it down ;D also, lulz, just the tip... 😂 In the case of potentially using silicone instead of tyvek, do you think it would be necessary still to cover the puncture, or would the silicone be self-healing enough to snap back since the gauge is pretty thin, as on a repuncturable medication bottle? Also, how do you think it would work using the sticky part of a waterproof bandaid and peel-applying it, since it was in a sterile environment inside of the packaging?

How long does it take to see any mycelial activity? Like weeks?

Can I heat up the tip, then wipe it with an alcohol paper towel, then inject it?

Are your lids upright or upside down?

In the fb groups they tell everyone to only inoculate grain with agar. Does it get contaminated often this way? I mean they preach this.

can we use coffee filter instead of tyvek?

Where should I keep the jars after inoculation? I also got a few pieces of vermiculite in my spore syringe. Am I able to save my syringe with a few pieces of verm in or should I just toss it? I prob have a couple of ccs left.

Thank you this is very helpful and complete

I have two holes in my jars. One for my syringe and another hole for air. This jar doesn't have a hole for air. I thought live tissue needed air to grow.

What grains did you use?

what top are you using ?

What grain are you using?

Lol, finally come to the point! I always splash off nicely XD

No hole for gas exchange? I would suggest using micropore tape instead!

Can you use aluminum foil for the lid of your jar?

Does it not need to breathe?

These magical

don't worry, it's just the tip...

the answer you came here for is 1ml of spore but he didn't say to how many ml in a container

Do you expect people to search all of youtube for it?

No air .

you may have already heard this by now, but lots of people are saying 70% alcohol is better for decontamination than higher percentages.

So I've heard that u r not supposed to knock up grains with spores....lol...idk why....ive had great success.

Do you have a degree in microbiology?

Need to use 70% alcohol 90% dries to fast

Part 4 is no fucking use. Wheres part 1

So many contams before you get that hole covered...

someone has a little sugar in their tank