My New Favorite Liquid Culture Recipe for Growing Mushrooms 

Funny story...been watching your videos and ordered all the stuff in this one so that I can start growing my own liquid culture. I just went on etsy and randomly selected a liquid culture syringe to get me started. I didn't even look at who the seller was until I had sent the payment. Turns out it was you XD

I've been using that same recipe for a couple of years now. It's the best imo. That peptone really makes the mycelium POP.

Thank you brother! Peace and love☮️❤️🙏🏻

I love how this comment section is basically a huge list of FAQs *that are being answered!* Keep it up, man. You've earned another subscriber.

Thanks!

Your results are fantastic looking, and I LOVE the simplicity of your recipe. Most I have read and tried are far overly complicated in comparison. I can't wait to try this out! Cheers!!

Been looking for this recipe, i believe this is the recipe used in the premade jars i bought.

I stuck spores from a MSS into coconut water as an experiment and it started growing myc after 4 days.

Spore n' Sprout is a great place to learn about fungi growing!🙂

Amazing ❤

I have been looking and looking for a product to buy or recipe for Water Clear Liquid Culture, not yellow... Its just a preference !! So Thank You !! This is the only one I found so far !!

I've been using this recipe for a out 6 months now, i use a little more peptone and malt extract but it looked like we get the same kind of results, as soon as you showed the ingredients I got excited, this is by far the best LC recipe I've used

Thanks for the easiest to follow video on liquid culture on youtube. Everyone else makes it super difficult. Or maybe I'm just not a smart fella?! 🤷 lol 😆

That is awesome I'm going to try it

LME is the best IMO! Clear and fast growth!

this video was cristal clear! ;D

The video is very easy to follow... thank you bro, from your guidance I made it. As a result, I get mycelium that floats lightly, so clean and smooth like a cloud in a solution that remains clear until the end of the process. Unfortunately I don't understand how to share with friends here. once again, I thank you very much..

After trying the original recipe with a 64th of peptone and LME and karo I just put together another 7 quarts of the suggested recipe for more nutrition. The first way shown in this video worked very well. I simply want growth for another round as fast as i can get it and plenty of nutrition to fill the jar with mycelium. The suggested recipe was 1G peptone, 1G LME and half tbsp of honey or corn syrup. I mixed the first quart that way and while stirring it i noticed it was definitely not completely clear. i lowered the LME to between.5 and .75 if a gram, not measuring it exactly just leaving the 1\4 teaspoon less than full and that solved the problem if the slight haze. Absolutely clear LC with that recipe. 1G peptone which is 1\4 teaspoon, .5 to .75G LME, light malt extract and half tbsp 7ml of Karo. I used karo only because that is what was there and honey seems to caramelize when i use it at 4% I have not tried it at lower concentrations, it might be perfectly fine. I wanted to list my findings for others who are trying to get completely clear LC but still wanting high nutrition. Thanks a million for all your help, especially the trick with adding 2 inches of water covering the jars in the bottom of the all American pressure cooker so I don't cause a haze after sterilization. It worked perfectly. I ran it between 12 and 13 psi after getting it to that pressure for 35 to 40 minutes and immediately vented. Perfect results. Thank you very much!

Thank you for your videos! They are very educational. I am more familiar with your old videos. Trying to catch up now. I feel my knowledge is a bit rusty after over a year break from studying mycology. I had experimented little and due to health conditions I was forced to have a break and my LC spoiled meanwhile. I’m ready to restart but now all I have is 1 spore print and 5ml spores suspended on water. Unfortunately I am not able to get more. I have materials for grains on jars, bags, agar, LC, bulk substrate… What would you recommend I start with so I make the best of the little I have? For grains I have access to rye and popcorn by the way. I am interested in starting a good self sustaining cycle loop so I don’t waste the spores I have. Your help would be greatly appreciated. Thank you in advance!

12 months!? Wow... trying this immediately

I use this same recipe…it’s the bomb. One gram of each to 600ml water 👍🏼

First of all, thank you for sharing all your knowledge! If a person wanted to run an exhaust fan a few times an hour for short periods of time, do you have any recommendations as far as a timer that is that precise? Thank you again!!

Thank you for making this video, have not tried it yet, but curious how to get the lc out of the jars to inoculate other thing! Thanks inadvance!

What flow hood are you using? Does it work well?

It looks like a lava lamp❤

I got your spores in the mail super fast, thanks. I'm about to make these mixes today, and eventually use them to mass produce shrooms in my 2400sqft shop. Let's goooooo

I have of the ingredients except for malt extract, amy other ingredients to sup the malt? Amd ty

Thanks for the valuable content. What amount of dextrose can be added instead of corn syrup?

I had good sterilizing in microwave for abt 5 min

I wonder even grinding down Morning Glory Petals to absorb in with Culture would help with Potency.

❤❤❤

Which model pressure cooker are you using? The 921, or 925?

My presto states to fill 3 quarts of water when using it.. does the LC recipe glass mason jar have to sit completely above the water during the 20 minutes or is it ok if the bottom portion of the mason jar is submerged in the water?

i love the clearness and very fast growth of this recipe. thank you! however, most of my strains now start to make significant rafts. i first thought it was contam, but surely they are not all suddenly contaminated. it gathers up in big floaters on top that is very hard to break. have you experienced the same?

This might be a dumb question but I'm a newbie so please don't make fun of me lol. Is it possible to take liquid culture that has already been formed, and inoculate another jar with it to allow it to grow? Essentially can you use one jar to make more jars? Also, on a side note, that is some absolutely beautiful looking liquid culture you have. Nice work! 👏👏👏🙌❤️

Hi, I appreciate your concise and simple instructions to create liquid culture. What is the suggested time to let the culture grow mycelium or is it by sight? (i.e. 50% of the liquid has mycelium) I'm trying to build up my culture backlog and extend my spore purchases. Thank you!

If I have light spore specs still floating do I keep it in inoculation longer and weight it out ?

So the idea is to sterilize the f2ck out of the jar and fill it with some liquid carbs? Would you consider trying a really crude version of this? Like wiping down the jar with some off the shelf isopropyl alcohol and then dumping some sugar water in with some innoculant? Idk I think it'd be cool to see if it works. Anyway, really cool footage.

Is there a reason you stirred with a frother initially instead of just using the magnetic stir bar?

when it is ready to fruit what do i use for the substrate do i have to use grain and inject it or can i sterilize a plastic bin with cardboard or something else please let me know

I wish you sold these jars on your website !!!! Lol I live with other people and can not make these, plus I do not have a pressure cooker... :(

I didn't see this and as usual I mixed everything in hot water, the peptone took a small amount of agitation to get it mixed but all three ingredients were very easy to mix in hot water. LME and I'm using light Karo just because that's what is here. I have honey but at 4% it badly clouded and a lot of sediment. I'm guessing I caramelized it with too much heat and too long of a temperature.

Thanks to these LC videos promoting this bacteriological peptone, Amazon is sold out. I decided to order the soy peptone instead. Given these results from something sooo diluted, we all need to promote this recipe so there's more peptone to go around as this mycological revolution ramps up 😉. Good to know at least that LC solutions can be successful over such a broad range of concentrations. I don't think I'll even bother measuring after watching this; just a couple of pinches of each to be sure I have at least this amount, but obviously waaay less than other recipes I've seen.

How much growth do you get after a week? Is the one you show with that much grow only after 7 days?

great recipe, can i replace syrup with dextrose?

😂 I just realized I brought liquid culture from you lol

Hello! First of all - amazing video and results! I would use some advice if you don't mind: I'm using 8 grams of fructose in 200 ml of distilled water. Sterilized the solution and inoculated it, kept the jar wrapped in the aluminium foil at 24-26 Celsius. It's been 4 days now and the only thing that appeared in the solution is a very thin and short transparent (or white, it's hard too tell they are so tiny) strings of supposedly mycelium. Also the spores (they appear as very dark brown dust) are doubled in size. Is it going well and I should proceed with waiting or the result I have now (4 days) is not enough and I should just scrap it and start all over again? Thank you in advance!

Great video and thanks. Thats 1/64 tablespoon. A teaspoon is about 1/8 of a tablespoon. So 4x that small one you got there or 1/4 of the big one (the size of a nail) would be a teaspoon.

can you please do a conversion to grams and milliliters?

Would using the mycelium in here be ok to use in an extract? Instead of like using the mycelium from rice?

What heat and light conditions do you sore the LC jar at immediately after inoculation?

Thanks so much, is it possible to convert the tea and tablespoon measurements into grams per 600ml?

Love this! ❤ But serious question : What kind of needle do you use to get to the bottom of this? Or do you fill the syringes with the lid opened in a sterile environment? Thanks!!

Is this better than your honey recipe for Cordyceps Militaris? I ordered some syringes from you last week and plan on making the LC before they get here. Thanks. Love your videos.

After you insert the syringes do you put the jars in the refrigerator?

Is this sterilisation is 30 minutes at 15psi? .should we Weight until the pressure cooker turns to 15 psi and the 30 minutes or all together 30 minutes from the minutes I started pressure cooking?

Hi what an informative video. thanks, I am making mine to your recipe and need to know what temperature to store the jar at?

I have a question . Do you burp your sterilizer , or simply allow it to run for 10 minutes before shutting the valve ?

What do you think about adding brewers yeast? Ive seen other recipies with this but im not sure what it does.

Thank you for this recipe. What do you think about using powdered corn syrup? Does mycelium have a preference of bone peptone over soy, or vice versa? Do you feel that this broth will work for pestilotiopsis microspora, initially anyway? We are excited to find out how this works with all of our different species. We have a number of projects going on both professionally and personally that this may be a nice addtion too. Thanks again for sharing it with is all!

Great video, like always! Is there a substitute for the peptone? I wanna try this recipe but in my country is not that easy/cheap to get!

You can leave it out in a cool room? Cool like like 65 to 70° ?

Ok mate, I bought some of your liquid culture from you, and have made this mix in this video. How much liquid culture would you recommend me using for each jar of LC?

Great vids, thanks, you would shoot spores into this ?

I tried this exact recipe which looked amazing and the only thing I didn't do was use your brand of malt. The corn syrup and the pep tone was the same brand from Amazon. I used Anthony's malt and it will not dissolve. Right now I'm not sure if it's the malt or the peptone but something isn't dissolving no matter what I do with the magnetic stir. They're the same color so it's hard to tell. And guess my only other avenue is eliminating one and then the other to see if they work at dissolving entirely in the liquid

after the mycelium growed enough, can the liquid in the jar replace the liquid cultures on your website? can we inject it directly into a grain to prepare grain spawn?

So I didn’t use an agar plate, but I haven’t used my spore syringe yet, so I still have time to buy one - how would I transfer it from agar plate to liquid culture ?

I dont have light malt extract but can i use only peptone and syrup?

if I'm using half gallon mason jar should I still PC at 15 psi for 30 mins or go longer than 30 mins? also if using spores syringe, how many cc should I use for the half gallon jar if I need fast colonization?

Question: I followed this recipe but I forgot to tighten the lids after I took them out of the pressure cooker. I left the foil on top though. I took the foil off 3.5 hours after removing them from the pressure cooker and noticed the lids were loose so I tightened them at that point. Do you think the jars could be contaminated? Let me know if you think I should start over.

Karo vs corn syrup ? Or what’s the difference ? Thanks in advance love the videos !

This week for starting mycelium grow I need to keep it in dark, worm place?

I am not capable of fully dissolve the ingridient and still look cloudy, any suggestion? I am using lme, bovine bone peptone and glucose syrup ( didn’t find corn syrup where I live)

ive had cultures last 3 years in a dark cool closet..........dont disturb the water wall inside and the mycelium will give off a digestion enzyme that sterilizes the water and essentially puts it in stasis.

Does this work well for any dung loving mycelium?

If myc appears flat floating at the top or sunk to the bottom is it dead?

Question so Ive seen online that people have said you only need a few drops of a spore syringe to 1ml. So what is the truth can you get away with less ? Or do you need more spores

How long/often should LC be put on the magnetic stirrer?

Can I use Jam jar for LC?

can i store it in the fridge or should it just be at room temp. After the mycelium has covered the jar and you want to store it.

nice tattoo. greeeeeeeat video brooooooooooo.

Questions about stirring: You say to stir once at the end and then let sit for a week. Then stir everyday after that so it doesn't form at the surface? How many days do you stir until? When do you put it in the cold room? Do you stir once it's in the cold room?

Can we use it for agar recipe?

Is that filter tape on the lid or is it completely sealed with the exception of the injection port

Once you got the mycelium grown and stirred you can store it for 12 months untouched or do you still stir it daily?

Do I need a vent along with the self healing port?

Is time for growth the same regardless of what mushroom you’re growing? For example I thought Cordyceps were ready after like 4 days tops?

can i use water from any machine ( water dispenser )

Hey amazing video!!! Quick question: does this recipe works with lion's mane mushrooms?

I've been using a recipe that uses about 1/2 tsp peptone and 1/4 tsp LME, but the same amount of corn syrup and water. You've found these much smaller amounts of peptone and LME to be superior to larger amounts like that?

is a magnetic stir plate just for convenience or can i just shake the jars

Do you know the measurements by weight for LME and peptone? Asking because I don’t have measuring spoons.

Is boiling tap water sufficient for this method or have you got any advice on how to distil water at home. Having a terrible time getting LC to work with honey currently so definitely going to give this a go!

My first LCs (3 jars of golden oyster, and 3 jars of lions mane) are 2 weeks old now and seem to have stalled. The golden oyster blob rises about 1/3 of the way to the surface, and lions mane is only about 1/5 or 1/4. This video inspired me to try a milder solution than most LCs recipes you find, although by my math, my recipe is still 5.5x richer than this (1/16th tsp each of DME and soy peptone per 350 mL jar). I'm also running 2 jars with a sloppy half teaspoon of honey added to make note of any differences, and so far there aren't any. Another difference is the fact I'm using a 0.22 micron syringe filter installed in the lid to provide the FAE. Do you suppose the syringe filter provides inadequate FAE for a 350 mL liquid culture in a 500 mL jar? I've given them a vigorous stir twice now. Do you think perhaps I need to stir more frequently, or for a longer duration? Or perhaps I'm just being impatient. What level of growth should I be seeing at 2 weeks?

is peptone necessary or can i just use malt extract?

Just to clarify will this work with spore syringes as well? not just liquid cultures.

where did you get your flow hood

Couple of questions. What happens if say 10% LME is used? and 2. How does the amount of LME affect the long term shelf life of LQ?

What is that red material that is used as an injection port?

My cl became cloudy how can in save ? I was using 1g of peptone