Preparation of nutrient agar plates l How to make agar plate

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In microbiology, it is very important to know how to make nutrient agar plates for routine microbiological work. Nutrient agar plates are prepared to grow microorganisms, especially bacteria.
Visit blog for more details: rbrlifescience.com
Hi, I Am Rahul B Rathod, Welcome to Our You Tube Channel RBR Life Science
About this video-
friends, In this video tutorial we are going to learn How to make nutrient agar medium plates and preparation of nutrient agar in petri plates for microbiology laboratory work thank you so much ....
Queries Solved:
1. How to make agar medium plates
2. How to sterilize media using autoclave
3. How to pour media into Petri plates
4. How to store agar plates in refrigerator
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#preparing agar plates in the microbiology laboratory
#how to make agar plates for bacteria
#preparation of nutrient agar plates
#microbiology
#prepare agar petri dishes
#Nutrient agar media preparation
#Nutrient agar media preparation in english
#how to make nutrient agar media plates
#Nutrient Agar
#make petriplates
#NA plates
#Media prep
#English
#rbrlifescience

Опубликовано:

8 ноя 2021

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Комментарии : 104

@modoumangan9851 Год назад

Awesome, very clear and easy to understand

@rbrlifescience Год назад

Most welcome, also please suggest any topic or concepts i should make such videos

@modoumangan9851 Год назад

@@rbrlifescience I will appreciate it if you make a video about putting bacteria broth into microtiter plate to measure their growth curve and optical density

@sindhujan5241 19 дней назад

The best explanation out there. you are awesome 💯❤ ❤

@rbrlifescience 19 дней назад

Thank you Dear

@greenworld1562 Год назад

Well explained

@Vjsnd Год назад

This is great for beginners in the lab like myself :) Loved it!!! Thank you

@rbrlifescience Год назад

Glad to hear that ... stay tuned to watch such more videos

@upeducationtec9064 3 месяца назад

thank you very much. well understood

@santoshbhavi8516 2 года назад

Thank you very much for this informative video.

@rbrlifescience 2 года назад

Most welcome sir

@himalayanwizard 2 месяца назад

Youve got infinite flask bro

@manand287 2 года назад

Thanku so much for u r hlep of my studies..god bless u

@rbrlifescience 2 года назад

Most welcome Sir

@soniyamourya8351 2 года назад

Sir really this video use for me👍

@rbrlifescience 2 года назад

Welcome Soniya ji ...You can also suggest topics and I will try to make videos on it 🙏keep it up

@lvs8850 6 месяцев назад

very good video

@rbrlifescience 6 месяцев назад

Thank you

@sawairagul251 2 года назад

Well explained 🥰

@rbrlifescience 2 года назад

Most welcome..And stay tuned

@viratkohli7137 Год назад

Thanks sir

@lovelyfeespanola7931 3 месяца назад

Why do some nutrient gar need to add sodium for prep?

@anassabduabdu9798 Год назад

Well done doctor

@rbrlifescience Год назад

Thank you and stay tuned to watch such more videos

@rbrlifescience 7 месяцев назад

I am just Master of Zoology.

@greenworld1562 Год назад

Your videos are very helpful. Thank you sir, plz make more video of lab experiment

@rbrlifescience Год назад

Sure, please suggest me topics or concepts on which I should make videos

@rulakakatipandey9554 Год назад

Nice and stepwise and easily understanding to all....thank u sir

@rbrlifescience Год назад

Thank you for complement..... Stay tuned for such more videos

@divya821 2 года назад

Sir make more how to reporting culture sensitivity and biochemical reaction

@rbrlifescience 2 года назад

Ok i will try

@user-ju3iw8lk6f 6 месяцев назад

Thank you ❤

@rbrlifescience 6 месяцев назад

Welcome

@Wrcher 4 месяца назад

Thank you

@rbrlifescience 4 месяца назад

You're welcome

@vlogwithsanu377 Год назад

Thanku 🥰🥰

@rbrlifescience Год назад

Most welcome

@poleenterprise7676 3 месяца назад

Thanks 🙏

@rbrlifescience 3 месяца назад

You’re welcome 😊

@sudiptakapri8624 9 месяцев назад

1.After making the nutrient broth we should check the the pH . 2. Before pouring the plates conical flask neck should be heated for contamination safety

@rbrlifescience 9 месяцев назад

Yes, correct

@ruchika_899 11 месяцев назад

Tysm ❤

@rbrlifescience 11 месяцев назад

Most welcome and stay tuned for such more videos

@ManiMani-qj8rb 11 месяцев назад

Petri art video panuga

@somisettyharshitha7821 2 года назад

Sir make isolation and identification of probiotic bacteria and yeast

@rbrlifescience 2 года назад

i will try

@arielbowmanoquinn7065 11 месяцев назад

I cracked up when he kept showing the petri dish pouring. 🤣 Classic.

@rbrlifescience 11 месяцев назад

Thank you that you liked it

@kolavinodha1242 9 месяцев назад

Sir can u make the vedio regarding the Isolation of fungi nd bacteria from spoiled fruits nd vegetables sir please

@rbrlifescience 9 месяцев назад

The process is more or less similar as explained in the video. You can use your sample instead of soil

@a110100 7 месяцев назад

Transfer a piece of the fresh fruit onto agar. Hopefully they grow separately and you can keep transferring away from the contamination.

@divya821 2 года назад

Sir make for culture sensitivity blood urine and so on

@rbrlifescience 2 года назад

ok i will try

@UyenTran-ut5sy Месяц назад

sir, why unused sterile medium should be then stored in the fridge rather than at room temperature condition?

@rbrlifescience 5 дней назад

The moisture from the agar medium will evaporate, and plates will become dry. For microbial growth to occur we need water in the agar plate. Also if we keep the petri plates outside then there will be chances of microbial contamination.

@kael3808 2 месяца назад

hi! what can we do if we’ll just sterilize the agar using pressure cooker? (we don’t have autoclave)

@rbrlifescience 2 месяца назад

Yes it will sterilize the agar

@HattoriHanzo62 Год назад

just a small and respectful comment: the correct measure unit to indicate "grams" is "g" and not "gm"

@rbrlifescience Год назад

Thank you for suggestion. I accept and I will definitely try not to write this gm for gram unit . Apology

@HattoriHanzo62 Год назад

@@rbrlifescience no need to apologize, 🙂everybody can make small imperfection: the quality of the information is great and I just wanted to help to be more precise

@rbrlifescience Год назад

@@HattoriHanzo62 👍👍

@rachealpalmer9941 2 месяца назад

Can I keep it for 48 hrs before refrigeration

@rbrlifescience 2 месяца назад

Yes off course

@ligayan5143 Год назад

sir how do u compute for the powder agar to be used?

@rbrlifescience Год назад

If you are using ready made nutrient agar bottle then there is no need for computation. If you are preparing by addition of individual components then agar percentage is 1.5% For making 100 ml of NA take 1.5 g of agar powder For making 1000 ml of NA take 15 g of agar powder For exact composition read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

@fansarulsk4415 Год назад

Blood agar macconkey agar video upload karo

@rbrlifescience Год назад

I got your request.. and I definitely going to make the video on blood agar and macconkey agar soon.👍

@nishitachitkara4623 5 дней назад

But why we use autoclaving or incubator

@rbrlifescience 5 дней назад

We use autoclave to setrilize and dissolve the media. After sterilization we pour the media in petri plate and after agar in plate solidifies we need to keep plates in incubator for drying for 24 hrs -48 hrs. This incubation is also check any contamination while pouring.

@RealKing213 Год назад

From where we can buy nutrition agar and can grow orchid seed

@rbrlifescience Год назад

You can buy the nutrient agar from various companies like Hi media, thermo scientific, Merck etc. Few are also available on Amazon

@winwinkhaing468 Год назад

Why you convert plates? Is it really need or not ? Please explain kindly me?

@rbrlifescience Год назад

After pouring agar media in the plates once agar media in the plate solidify (usually after 1-2 hrs) then it should be kept in inverted position. Now two things are there, lid and plate with Media. If we invert the plate then due to weight of media in plate kept on lid there no air will enter inside the plate. If we keep the plate as it is after pouring then there are chances that air will enter inside and plate will be contaminated. This is the reason that all solid agar plates are always kept in inverted position in order to avoid entry of air inside the plate. Also plates are not contaminated while handling in inverted position.

@gardeningonly 4 месяца назад

Which is the good online platform to buy nutrient agar for india?

@rbrlifescience 4 месяца назад

It's himedia labs

@gardeningonly 4 месяца назад

@@rbrlifescience thank you i already checked there but they don't have 100gm I am just learning from your videos and blog so just want to try with small amount.

@rbrlifescience 4 месяца назад

You can purchase from Amazon you will get 100 gm

@bernadettegaby Год назад

For 23 gm of nutrient agar powder, add 1 L of distilled water. Is it right?

@rbrlifescience Год назад

yes correct, whatever media you prepare there is information available on bottle itself. Take 23 gm of nutrient agar powder add it in 1000 ml distilled water

@hak8278 Год назад

28 mg for 1L👍

@rbrlifescience Год назад

It will depend upon which company media you are using. In this video i have added 28 grams of nutrient agar in 1 litre of distilled water. Instructions are always written on media bottle itself

@zesseljhontaguibolos6621 Год назад

whathappen do them after 24 hrs, 42 hrs and 72 hrs?

@rbrlifescience Год назад

As the time passes the water in the agar gets evaporated if we keep plate in the incubator. A time comes when the agar falls from the lid after 5 to 6 days

@soniyarajkumari9419 6 дней назад

How do we know that we need 4.6gm of NA in 200ml of H2O

@rbrlifescience 5 дней назад

On NA bottle, it is mentioned that for making 1000 ml of NA you need to take 28 grams of NA powder from bottle and suspend it in 1000 ml distilled water. It's just calculation 28 g for 1000ml 2.8g for 100 ml 4.6g for 200 ml Have a great day 😊

@Syedatalhanaqvi 3 месяца назад

But he didnt use sand for bacteria ??

@rbrlifescience 3 месяца назад

its animation for understanding

@_liah_hussain_ 2 месяца назад

Because he's using nutrient agar powder.

@TechsScience 2 месяца назад

I tried this Experiment but got no reasult Also my solution looks whitish

@rbrlifescience 2 месяца назад

After autoclaving solution will be clear

@TechsScience 2 месяца назад

@@rbrlifescience but the agar solution is yellowish In general My solution remains pale yellow even after autoclave & yes I am taking an edible agar (used to make jelly) is it OK?

@rbrlifescience 2 месяца назад

Once agar in plate solidifies then it remains slightly yellowish. Nothing to worry about.

@TechsScience 2 месяца назад

@@rbrlifescience it's not about the color the problem is I am not getting bacteria culture even after 7days

@niloofarjadidi6030 Год назад

hi, what is the percentage of the ingredients of nutrient agar media?

@rbrlifescience Год назад

Nutrient agar media has different components such as Peptone, Beef Extract, Sodium Chloride, and Agar. For exact composition percentage please read our blog post rbrlifescience.com/nutrient-agar-media-composition-and-uses/

@chkumaryadav657 Год назад

Nutrient Agar 37grams per 1liter so potatto Dextro Broth 24 Grams per 1 liter

@rbrlifescience Год назад

Whatever media you are preparing there is information available on Media bottle itself. Generally there is information given for how much media you should add to make 1 litre solution of that media .

@chkumaryadav657 Год назад

@@rbrlifescience ok sir good information

@prabhadabahde1905 2 года назад

Sir humko 17 g Lena hai kaise weigh kare

@rbrlifescience 2 года назад

First take a weighing boat or dish and keep it on digital weighing balance and tare the weight of dish to 0 . Now open the nutrient agar powder bottle and add the Nutrient agar powder in dish till the weight on weighing balance shows 17 gram.

@prabhadabahde1905 2 года назад

@@rbrlifescience thank you sir

@rbrlifescience 2 года назад

@@prabhadabahde1905 most welcome