Slants vs Plates explained: mycology tips and tricks 

thanks it’s how my brain works - The content is all related but not linear like most tutorials and I post in real time so it documents success and failures which is important to show people

At first i felt it was scattered, untill i learned something about mycology, then my opinion changed to segmented. Lots of great stuff when you know what to look for.

thanks for following along we are glad to help the industry move forward MUSHLOVE

@@FreshfromtheFarmFungi way to ignore the other guy

Would you be willing to sell me a 25 pack so I don’t have to buy the full case of 500? 😬

It is hard to do with larger bags but I like using grain jars because it’s easier to hold them with one hand while the other hand controls the top of the bulk bag - hope this helps!

thanks for watching and following along! 🍄❤️

Thanks for watching! Yes on all those questions we let the culture grow out 80% or so and then refrigerate 34-37F

@@FreshfromtheFarmFungi Amazing, thanks Gary!

yes the cooler temps will slow the growth - it’s a continuous struggle! Lol just make sure to have backups and you will be fine

@@justinlangley9529 thank you, that link lead me here: www.amazon.com/SPL-Conical-Centrifuge-Racks-Sterile/dp/B01M04HGPJ/ref=pd_sbs_328_1/133-5604448-1900151?_encoding=UTF8&pd_rd_i=B01M04HGPJ&pd_rd_r=185e994b-e8d2-4b4e-9305-7b5ec976e22f&pd_rd_w=VhlGU&pd_rd_wg=NftlA&pf_rd_p=ed1e2146-ecfe-435e-b3b5-d79fa072fd58&pf_rd_r=44RNG7X44VBY27TWHDVE&psc=1&refRID=44RNG7X44VBY27TWHDVE

yes those ones work great! just make sure you get sterile ones

it wont let me sign into amazon.com even though I have an account on amazon.ca so I cant buy those.....

We use MEA (Malt Extract Agar), PDA (Potato Dextrose Agar) and V9 Agar (V8 + Potato Flakes)

@@ZeginMakesMusic nice work 👍 before pressure cookers and fancy flow hoods people were able to get success this way. It can be done and is a good building block for technique

fridge at 37-38F is ideal for most species - some are cold sensitive like agaricus blazaie

years or even decades depending on the strain, media, sterility etc.

either mea, pda or V9 I like to rotate the recipes with the agar plates to keep things fresh (14-15g/L agar and 24g nutrients)

@@FreshfromtheFarmFungi is this for 500ml? And when you say 24g nutrients, what do you mean by that? Can you please provide me with a list? Kindest regards

we make them ourselves - the tubes are sterile centrifuge tubes from “NEST scientific”

Thanks for the info! It is a common practice I just outweigh the benefits of time putting them in each slant vs. effectiveness and choose not to put them in - just preference 🙂❤️🍄

I appreciate it we are looking into couriers that will do international but there are more regulations with living mycelium

And me and me and me

hah yes there are many “live and learn” scenarios in the lab but then you move on 😆

A ton of plastic is saved utilizing LC’s and glass media jars which is a revolutionary process that we utilize on our farm.

Thanks for the algorithm boost! Im from Buffalo, went to school for laboratory technology, worked in labs for years, supervised labs - it is how it was taught to me - sometimes people have accents and saying it how I say it in fact means the same thing

@@FreshfromtheFarmFungi explain it away all you like. I dont care where youre from, how many labs youve worked in, or how ignorantly they (and you) pronounce it. Its simply wrong. It is most definitely pronounced "ay-gar" the world around, even in different languages. Moreover, It most definitely does NOT mean the same thing. An "auger" is a tool with a helical bit for boring holes in wood. It would behoove you (and anyone else saying it this way) to learn how to properly pronounce things, most especially in an educative setting. It would also behoove you to learn to take viable constructive criticism from your viewers. But you do you boo, youre apparently the expert. What do we know, right?

@@FreshfromtheFarmFungi it is Agar.it Is not an english word like they are trying to tell you. Don’t pronounce it like “ay gar “ plz. Pronunce it just like when you say Agaricomycetidae. You are right. Sinceramente una persona que habla español :3

Oh we got an English teacher in the house. Your getting free information here. If you don’t like how he talks then get to stepp’n!!!

@@lhicks1354 ok sad sack. I dont need a free education, this video came up as an autoplay after another video I was watching out of boredom. Ive been pouring plates and slants for over 2 decades, specifically and only for mycology. I have also lived in many countries over that time: Germany, Italy, France, Venezuela, Guatemala, and the US. I speak from experience, experience in several countries that do not speak English, yet still pronounce it the way I suggested he should. And, maybe youre ignorant on many levels, because you obviously do not understand how public forum works. People are not only allowed, but should be encouraged to educate each other, especially on matters of science. You should "get to stepp'n" right into a science glass, and shut your ignorant pie hole 😉

yes it is best practice to screw it down tightly. The point is to slow growth to an animated state which does not require gas exchange so it can be refrigerated and “woken up” later. There is enough air in the head space of the tube for the mycelium to survive

@@FreshfromtheFarmFungi thank you, your videos are amazing my man.