Basics of chromatography | Chemical processes | MCAT | Khan Academy

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Understand the basic principles of different kinds of chromatography: paper, thin layer, column, size-exchange, ion exchange, affinity, HPLC, and. By Angela Guerrero. . Created by Angela Guerrero.
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Опубликовано:

16 сен 2013

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Комментарии : 273

@trilliumchang4127 8 лет назад

The point on size chromatography is wrong. Larger size elutes faster as it can go around the beads, whilst smaller beads have greater retention as it has to go through the intriciate pores.

@metoo2here 10 лет назад

Hi..the video is great..but you have made a mistake with size exclusion chromatography..the molecules that are larger in size (the ones excluded from the beads due to larger size) are the first ones to elute out followed by the small, the smaller and the smallest..the reason being that the molecules which can enter the beads via the pores have a longer path and hence a longer transit time.

@rhaziongames3696 4 года назад

Sal is better

@nihadxo7160 2 года назад

this badly explained made me more confused

@user-ee7uc9ki8u

The size-exclusion chromatography is wrong. Larger molecules elute FASTER than smaller molecules due to the small molecules being trapped by the pores in the beads.

@vistian 2 года назад

You're going to cost people points on the MCAT with the misrepresentation of size-exclusion chromatography. The larger molecules elute first.

@hafsa1698 9 лет назад

What happened to Sal's voice? 0_o

@kristak.1625 5 лет назад

everyone is either 1) sexualizing the speaker 2) criticizing and comparing the speaker to Sal... what does that even say about you guys? Lovely video, they're just jealous you understand chromatography and they don't.

@nobodyjames5804 5 лет назад

Guess who’s not going to fail her chemistry exam tmr

@carlospereira6183 2 года назад

Two words: Castor beans

@CORPSE77 6 лет назад

* correction, it should say: in size- exchange chromatography, small particles get trapped in the column by the small beads, thus small particles are slower to move. I just read that in Kaplan MCAT biochem review.

@oliviadenyer-dobrowolski7441 7 лет назад

Shout out to you! Thank-you for your simple yet complex explanations because I completely understand this topic now and feel confident to write my extended response!