Performing Column Chromatography

Подписаться 3,1 млн

Просмотров 76 тыс.

50% 1

We just learned about thin layer chromatography, but a much more industrially relevant technique is column chromatography. This is how we will separate large quantities of compounds in a mixture by virtue of their differing polarities. It follows the same principles as TLC, but we are pushing compounds down a column of silica gel rather than up a TLC plate. Let's check it out!
Visit Thermo Fischer Scientific for all your laboratory needs: www.thermofisher.com/lab-chem...
Follow Jamal Muhoza on social media: linktr.ee/dr_Jamal
Watch the whole Chemistry Laboratory Techniques playlist: bit.ly/ProfDaveChemLab
General Chemistry Tutorials: bit.ly/ProfDaveGenChem
Organic Chemistry Tutorials: bit.ly/ProfDaveOrgChem
Biochemistry Tutorials: bit.ly/ProfDaveBiochem
Biology Tutorials: bit.ly/ProfDaveBio
Classical Physics Tutorials: bit.ly/ProfDavePhysics1
Modern Physics Tutorials: bit.ly/ProfDavePhysics2
Mathematics Tutorials: bit.ly/ProfDaveMath
EMAIL► ProfessorDaveExplains@gmail.com
PATREON► / professordaveexplains
Check out "Is This Wi-Fi Organic?", my book on disarming pseudoscience!
Amazon: amzn.to/2HtNpVH
Bookshop: bit.ly/39cKADM
Barnes and Noble: bit.ly/3pUjmrn
Book Depository: bit.ly/3aOVDlT

Опубликовано:

9 мар 2023

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 58

@sjzara Год назад

I was shown around the department at the start of my biology Ph.D. first thing I did in that laboratory was accidentally knock over someone’s column chromatography experiment.

@entropy-1 Год назад

It is ok friend we are all human

@VaughanMcCue Год назад

Prof Dave warned you to use TLC. Lab gear has feelings and needs tender loving care.

@JamesVestal-dz5qm 10 месяцев назад

Oooooh bad!

@watchapgang7150 23 дня назад

пиздец

@caseyr803 5 месяцев назад

Oh my god. I am currently a PhD student in ochem and I didn’t actually learn what a column is (my undergrad was not quite on the level of most institutions). My current research lab has an auto column but I’m also just very early in my research so I’ve only used it once and don’t quite understand the theory if it. This is my first time seeing the theory and it truly reminds me so much of why I love chemistry. So interesting!

@martinarenzi4744 Год назад

Thank you! I am so happy that you made this playlist. I am looking forward all the spectroscopies and mass spec. I love to see all the lab procedures through theese videos and their applications in real life analyses. Thank you Professor Dave ❤

@borttorbbq2556 Год назад

It’s because of you and a few other chemistry channels I follow but I do actually get home ads for lab equipment pretty often

@stephenwalton8507 Год назад

Interesting video. I used to run flame ionization chromatographs to analyze hydrocarbon gas samples.

@Thaumius Год назад

One point to add, please dont manipulate silica without a respirator, its a inhalation hazard, especially doing the wet technique.

@waelfadlallah8939 Год назад

Thank you for showcasing the basics of this technique and ending by displaying the latest advancement in this field :)

@crickerloverwithlove 3 месяца назад

Class 11th experiment teacher said do it your self in lab now I understand this❤. Thanku

@RafaelSilva-by7yz Год назад

Hello teacher, how are you? Could you explain in detail, if possible, how to select the best column based on silica density? Thanks for the video

@Goose-ib2ti Год назад

Good stuff. You should do a video on GC!

@martinlam5161 Год назад

May I know the type of silica gel used in this video, please? Thanks!

@paulmoran7026 Год назад

I’ve done 100s , possibly 1000s of columns, but nothing beats doing cryogenic columns over 24-48 hours with sensitive precious metal compounds.

@matte4123 11 месяцев назад

lysergic acid is much more fun

@wangchuanqi3942 Год назад

I usually prefer to add sand before I load the sample. It is less likely to disturb the silica gel level with sand on top.

@ashigaruvolt6548 Год назад

Thermofisher sponsorship? Let’s go

@anub492 29 дней назад

good one❤

@erikamogelvang6171 3 дня назад

URGENT question, 25 mL of solvent was added to what mass of the silica gel powder?

@user-lo1hj3zo3n 8 месяцев назад

Thank you. your videos are very useful and interesting. just please speak in lower speed. so that people like me that English is their second language will understand better. Thank you Professor.

@Artavazd.kirakosyan 10 дней назад

thank you very much. quick question: the solvent that is used to dissolve the compounds (6:59), is it same as the eluent?

@Noldor97 9 дней назад

So you could do that. keep in mind that you really want to use a solvent where your sample is fully dissolved in as little of solvent as possible.Most of the time it doesnt really matter if you use some other solvent here, as its getting diluted so fast in the column that it doesnt really matter.

@Artavazd.kirakosyan 9 дней назад

@@Noldor97 thanks a lot.

@rashaselem Месяц назад

شكرا لك

@lucidity1 Год назад

that's cute, when do you discuss HPLC? it's my favorite.

@eliottjung4568 Год назад

Solid loading team

@defeatSpace Год назад

Fun stuff!

@JamesVestal-dz5qm 10 месяцев назад

Lamont taught me that the first channel you turn on in the morning ought to be your occupation!

@GhulamAbbas-ci5zz Год назад

Start a paleontology series

@pahadipreet5812 9 месяцев назад

Thanku 😊

@stephan5279 Год назад

Ohh... Column Chomatogaphy... The fear of every person which does synthesis...

@Noldor97 9 дней назад

what do you mean? ding columns is great:D

@manthandambhare7395 9 месяцев назад

Awesome

@rassimsimou1594 Год назад

Good

@WKUConsulting 2 дня назад

may I use pieces of this video for a youtube training seminar? I will of course credit you and fischer. as well as link your channels.

@ProfessorDaveExplains 2 дня назад

Yeah if you're just playing the video on a RU-vid player of course

@WKUConsulting 2 дня назад

@@ProfessorDaveExplains thank you!

@colinprice712 Год назад

Please, please, don’t just turn the tap on any burette with one hand! You can put a lot of strain on the thin tubing joint between the tube and the stopcock. If it’s just a little stiff you can break the stopcock off spilling solvent everywhere let alone the risk of glass fragments… And yes, that’s from experience…

@nirmalmishra6404 Год назад

Sometimes you are Badass, sometimes Dick, you are non other but professor David.

@maheraberu2000 Год назад

The problem is that producers of beads like thermifischer unfortunately don't say what is inside the slurry of the beads and that makes sometimes problems. It's extremely expensive too

@ProfessorDaveExplains Год назад

Of course they say what is in it. Why would you think that people don't know the composition of silica gel?

@ObichereGolden Месяц назад

Please what is the need for the sand?

@Noldor97 9 дней назад

Okay so: sand at the bottom to prevent silica from getting in your samples that you collect. Sand at the top to prevent to disrupt your silica when you are readding your eluent

@JamesVestal-dz5qm 10 месяцев назад

Boy you can't get no higher than this!