Biochemistry | Michaelis Menten & Lineweaver-Burk Plot

Подписаться 3,1 млн

Просмотров 317 тыс.

50% 1

7:43 Correction: All the curves start at the "Origin" of the graph where the axes intersect, like in these illustrations of the whiteboard at drive.google.com/file/d/16NfK...
Official Ninja Nerd Website: ninjanerd.org
Ninja Nerds!
In this lecture Professor Zach Murphy will continue our discussion about Michaelis Menten and talk further about the Lineweaver-Burk plot. We hope you enjoy this lecture and be sure to support us below!
Join this channel to get access to perks:
/ @ninjanerdofficial
APPAREL |
www.amazon.com/s?k=ninja+nerd...
DONATE
PATREON | / ninjanerdscience
PAYPAL | www.paypal.com/paypalme/ninja...
SOCIAL MEDIA
FACEBOOK | / ninjanerdlectures
INSTAGRAM | / ninjanerdlectures
TWITTER | / ninjanerdsci
@NinjaNerdSci
DISCORD | / discord
#ninjanerd #MichaelisMentenEquation #LineweaverBurkPlot

Опубликовано:

25 апр 2017

Ссылка:

Скачать:

Готовим ссылку...

Добавить в:

Мой плейлист

Посмотреть позже

Комментарии : 142

@con1905 Год назад

I'm taking a biochem class in college, I haven't learned a single thing from my lectures and you're saving me right now. I can't tell you thank you enough!

@10knovideoschallenge32 5 лет назад

Sir ! you have an error ! you started the graph from Km ! the graph should start from 0 whatever was the type of inhibition, Km and Vmax are changing but the enzymatic reaction always start from 0. Thanks for your efforts and hardwork ❤

@Abi-jb8qt 5 лет назад

10k No Videos Challenge I think so there should be another line inthe graph which starts at the point 0 then goes and joins vmax line,but this line should be a little bit more curved ,so the Km will be higher and Vmax/2 will stay at the same point.(Cause vmax isnt changed)

@fatemeh4266 4 года назад

Yes! I wanted say that but didnt know how to explain

@litedelight7391 4 года назад

Yup, curve always starts from zero just area under the graph increases or decrease in each Type of inhibition.

@samianadir1356 3 года назад

i agree,

@eisenj21188 3 года назад

I read the comments before watching and now I understand what you guys meant. Thanks! still a great video!

@faizajilani993 3 года назад

never seen someone explain such concept with so much clarity...like he was made for this

@sheefalydigal9753 2 года назад

Yo, he's a doctor though.....

@eidleabdirashiidomar9499 2 года назад

Yeah

@MissBenjii 6 лет назад

Im a student from Sweden and I can only say THANKS to you!! You've saved me!

@thearchive8687 2 месяца назад

Thank you, Zack! Very comprehensively explained and yet easy to understand. This channel is one of the few that makes core concepts in biochemistry easy to follow. I’m reading Devlin’s biochemistry in sync with your videos. This channel for me is not just educational or informative which of course it is. It’s the ideal that you follow in taking such care to put top notch lectures out there that keeps me coming back nearly everyday at least in recent times. I’m planning on finishing all the lectures in a few months.

@crisseltomas5936 2 года назад

im speechless. thank you so much for explaining everything so well

@ammuponnu9601 3 года назад

Sir i have no words to thank you, sir its very easy to understand you are amizing 😇sir iam from india from kerala state, mostly i cant never understant the foregin accent bt yr language is understandable nd easly to follow, thanks for being there to help us, 🙏😇

@maxruphio5515 6 лет назад

I just love your vedios, highly detailed and love it how you connect different concepts.

@drreyes4432 Год назад

Best video ever, the only source that made me understand everything perfectly! thanks

@nihadzerroukhat0433 4 года назад

You'r amazing u just made biochemistry easier for me Thanks a lot 😍💜🤗🤗🤗🤗 Looking for more

@majosimpara9710 2 года назад

You are made for this job ! Thank you !

@przhadana9729 4 года назад

You video is very very helpfull ,thank you so much ,but i have just a small suggestion ,please after the lecture take a picture of all the board then put it here 🙏🏻🙏🏻 i want to study them😊 i hope the best for you❤

@malinamorales6527 2 года назад

What impressed me the most with this entire video was how straight all the lines were.

@user-bi6yk5wg9b 3 месяца назад

😂😂

@smit5934 Год назад

Superb! I was really struggling with this concept Now I know the key is to convert the graph into Km and Vm

@muhindoedith5459 2 года назад

Thanks alot. You make everything very easy to understand

@ranulidassanayake3171 3 года назад

Thanks alot.You explained everything really well.Can you please explain y Km increases/stays the same (variation of Km) depending on nature of inhibitor?

@joec3168 3 года назад

wow, how much the last 45 years would have been if I had you for study aide in college! relearning if so much fun !!!!

@drrahimullahkhattak5379 5 лет назад

really nice lecture i understand throughly very nicely thankyou so much

@user-vm9mv7tu9i 4 месяца назад

In class I used to sit at the end and watch your lectures😂 Thanks to you❤

@andreidan2878 Год назад

Question! Since the Km is the concentration where half the Vmax is reached, why do the lines for all the inhibitions START at Km? I feel like they should all start at zero and it should be the slope of the curves that pushes the Km to the left or right!

@galileerwamiye3669 6 месяцев назад

it might be too late to respond to your question but you're right all slopes should start a 0 because when there is no substrate there is no rate of the enzyme. It needs a substrate to operate.

@margheritagentile7846 6 лет назад

Your lessons are amazing, a big GRAZIE from Italy

@mushahidsalmani8 5 лет назад

Amazing ! Thank you so much.

@refad333 4 года назад

very interesting and easily described lecture

@rohamhadidchi1196 Год назад

Simply remarkable

@imaneaya4833 Год назад

جزاك الله خير Thank you so much sir

@VibeMD 2 года назад

I feeL the graphs are incorrect since while explaining the initial graphs you made Km the starting point, instead of it being where the axis meet

@claytonadkins2304 10 месяцев назад

I agree

@risconsia 9 месяцев назад

initial km is for the substrate,the ones in colored ink are for inhibitors so they shift right

@bartgertsen6181 9 месяцев назад

@@risconsiayes but why does the graph start at the km

@pizzamargherita9628 9 месяцев назад

@@risconsiano, you’re incorrect. Km is the substrate concentration which will give us half of the maximum rate of reaction (velocity) for that specific enzyme. You can’t start at Km. You start at the origin, and then shift right going for your Vmax. Then you can find the new Km in that line. But you can’t use Km as the starting point. So the graph is incorrect, I think he just didn’t notice.

@drids. 8 месяцев назад

@@pizzamargherita9628I agree with you mr pizza

@MaymoonahAslam 7 месяцев назад

omggggggg thanku soooo muchh you're really a very good teacher it means alot to meee thanku so muchh stay blessed ..

@lucasmuss5098 Год назад

Incredible video!

@vjhambh4691 3 года назад

It is really wonderful. Please make video explaining microscopy light and electron microscopy

@ME-ue9ss 2 года назад

hey zach thank you very much it helps me a lot i am happy to know you god bless you genious thank you very much

@ssenogaJonathan 9 месяцев назад

This man from another planet

@BioScienceClass 4 года назад

More Videos on Lineweaver Burk plot: ru-vid.com/video/%D0%B2%D0%B8%D0%B4%D0%B5%D0%BE-wAWYIGTRXQE.html

@vid6149 3 года назад

Great work sir

@lilyjoyeux6392 4 года назад

So thanks for turning something difficult in a something easy

@Outono937 6 лет назад

Your classes are brilliant!!

@NinjaNerdOfficial 6 лет назад

+daniela andrade coelho thank you so much for your kind words I'm so happy that we were able to help!

@sademthunasi775 Год назад

You just saved my life😊😊

@MandeepKaur-uv5tn 6 лет назад

Thanks once again 😊

@vampcreations7470 5 лет назад

Superb sir 👌👌👌

@muzamilnazir624 3 месяца назад

Brilliant Ninja Nerd

@bodyspeakswithmusic1862 3 года назад

Sir if v max decreases then that red poin of y axis will b lower from the pont u mentioned

@forever6890 3 года назад

really useful thanks alot legend

@drawwithnannu4562 5 лет назад

Thanks again .

@michaelmedicforreal8 2 года назад

I'm speaking French I'm studying in Italy and I would work with you for helping me to buying my study. I'm able to translate ours video in Italian and French language. 😊 thank you so much

@solomibarium324 Год назад

Thank you Sir

@badhmapriyadevarajan1725 Год назад

hello nice explanations, i have doubt that why we have to plot lineweaver bruk plot while we are getting vmax and kM from micheal -menton plot

@Maya_Alshabk 20 дней назад

you help medical students across the world :) greetings from Germany

@yamifonseca2993 6 лет назад

Great video!! i needed to slow down the video. But is because im not native english speaker.

@CORPSE77 4 года назад

I agree, he speaks very fast even for a native speaker. I have to pause the video a lot so I can comprehend what he says :). Keep in mind, he is speaking the language of biochemistry, which is a very dark language .. lolzz

@thesanode5285 4 месяца назад

Thank You!

@kamillamiller7234 6 лет назад

Thank you!!!

@NinjaNerdOfficial 6 лет назад

You’re welcome!

@malithaniman1730 4 года назад

Helpe a lot..thanks

@aswingrace6980 9 месяцев назад

Thank you 🙏🙏🙏🙏🙏🙏

@ayalemfarrak7691 9 месяцев назад

Could you please do a video about allosteric enzymes ?🙏🏼 Thank you for your efforts 🌹

@giuliodebenedetto-qz7zj 8 месяцев назад

What you don't understand about allosteric enzymes? I can explain it to you maybe

@jinx2873 2 года назад

thank you

@niloofarkh4779 Год назад

thank u so much

@Erika-ug8vm 3 года назад

Shouldn't the inhibitor lines start at the origin?

@goddesssmiley4304 3 года назад

Thanks

@OluyaliSeth 4 месяца назад

quick question: on the graph drawn on the 9th minute 28 second shouldn't the graph start at the origin and cross the half Vmax dotted line at the same point and normal then not make it to Vmax ? so as to bring out the idea of no change in Km clarify on that

@gideonibekwute 3 года назад

Thanks🙌🙌🙌🙌

@JO-2002 Год назад

BRAVO 🎉

@moizvahora241 Год назад

Amazing

@user-hp7tw3ge7s 3 месяца назад

I don't understand😢, if the km increases which would lead to a corresponding decrease shouldn't the graph tend towards zero given that the km is negative and the decrease once in the negative becomes bigger?

@sofiiakutsenko Год назад

how did we get -1/km on x axis in lineweaver-burk?

@wabisabi4717 Год назад

Where did the -1/km come from in the 2nd graph?

@user-ij8si3kr6b 6 месяцев назад

hey, i don't know if I understand it wrong. But at 10.14 he draws the line for N.C.I. the Km stays the same, but the km is not the start of the line right? It's when it's 0.5Vmax right? Now in the graf the Km of normal and non competitieve inhibition isn't the same.

@e_220_nourmuhammed5 Год назад

If we need to extract invertase enzymes in the lab from saccharomyces yeast and give the sample for 3 students A,B and C , they extract in the same way Under the same conditions and the same chemical student (A) get 3*Km ،Student (B) get 2*Km , Student (C) get 1*Km , So, who extracts the highest amount of invertase ?

@salimsikder2364 6 лет назад

Dude, i have a huge confusion on that point, why are you starting the graph from the increased value of Km on x axis? shouldnt the graph still start from the zero (all three case)? because Km = [S] in half Vmax, help me please!

@anonymous672 6 лет назад

Salim Sikder you sir are right

@hebakhalid857 5 лет назад

Same question 🤔🤔🤔🤔🤔🤔🤔

@hebakhalid857 5 лет назад

Salim Sikder same question

@MelanieGloria 5 лет назад

Obviously hes not so good at doing graphs.... anyway still very grateful

@nish209 5 лет назад

This is actually the first blatant error I’ve witnessed after watching so many of his videos

@haleemaazhar4393 3 года назад

Thanku sir

@03-eshanair38 4 года назад

The t should be written in small or big

@vudat189 4 года назад

km increase but what if it in 0.001 to 0.1 ? 18:04

@gypsypikis 6 лет назад

Thank youuuuu!!!!

@NinjaNerdOfficial 6 лет назад

You’re welcome!

@malikaalibdat7079 2 года назад

Line weaver -Burke plot begins at 12:57 Thank me later

@syloh5534 Год назад

@laromkashy3136 Год назад

What about Irreversibile Inhibitors? You talked about them in the last video but never mentioned if the change and how they change the Vmax or Km. Can someone help me understand it?

@humayra4277 Год назад

Because the substrate can’t bind to the enzyme, both Vmax. The substrate being unable to bind to the enzyme means no ES complex forms (affecting Vmax); the enzyme would have a much lower affinity to the enzyme (binding to the inhibitor) and I’m pretty sure it would mean Km is higher. Higher Km = lower affinity

@humayra4277 Год назад

Vmax would be lower, I meant to say

@laromkashy3136 Год назад

So Lower Vmax and Higher Km Are you sure? Cus I have been reading and it seems to be exactly like a competitive inhibition

@humayra4277 Год назад

@@laromkashy3136 with competitive, with enough substrate you can eventually knock the inhibitor out. But because this one is irreversible, the inhibitor can’t be knocked out, and so you can’t form the ES complex

@NaasiseeDaniel-ef4rh 6 месяцев назад

Why is the caption is not working🤔

@user-ju5tg3wl6r 6 месяцев назад

I need to send a hard question and solve it before my exam today please before 10 AM could I?

@ChAbdurehmanGujjar 4 месяца назад

Great great🎉🎉🎉

@billymeeks845 Год назад

SOLID.

@mattulbing 3 года назад

I really appreciate this channel, though I would very much like to see this video redone with the MM graph all beginning at the origin, the uncompetitive inhibitor points placed according to the direction the Km and Vmax indicates they should, and perhaps a yardstick and more space between the changes in intercepts to emphasize the change in appearance of the lineweaver-burk plot's lines. Thank you for your explanation, it just took me watching the video about 10 times to put my finger on why I felt like I was understanding it here, but there being variance from other sources.

@sarmadghafoor1484 4 года назад

18:05 He uses the force to lift his cam up exposed.

@sofiasjostedt9516 Год назад

Can you describe Kcat to me?

@AliAhmad-zn9vc Год назад

17:55

@UnityAxist 3 года назад

Vmax doesnt move

@imanesaouli9880 3 года назад

can any one show me how we calculate enzyme activity??please!!!

@alimuddin-hf2ez 3 года назад

You can use spectrophometer

@UnityAxist 3 года назад

Vmax thoes change

@michaelfuxeklint4909 2 года назад

I've got bad news for you, we're going to spend a lot of time together the coming year 😁😁🤭

@Dedded404 4 года назад

What about a mixed inhibitor?

@albertodanieldelapazayala6633 2 года назад

Algún comentario en español

@nihalihsan2688 6 лет назад

Superr👍👍👍

@junaidbhat818 3 года назад

Incorrect graphical representation of enzyme inhibition. It starts from 0

@simonemarciano6588 3 месяца назад

i love you

@dostmehmood3986 3 года назад

💓

@dhritidasstudy9337 2 года назад

👍

@firuddinabdullayev18 3 года назад

👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼👍🏼.

@eveberry9215 4 года назад

MARRY ME

@medicoimperfecta Год назад

Qasam se haya jese logon ka to moh torr dene ka Dil krta ha . Mtlb Kuch bhi ? Koi ghairat, koi sharam ?😂😂

@manyworldsin1 3 года назад

Uggghhh! I live this guy sooo much. He explains things so well, but this video is MEGA cringe. The definition of asymptotic means the curve never reaches it. AND WORSE! He keeps starting the inhibition curve with an x-intercept at the uninhibited K(sub m) and saying the K(sub m) stays the same. That is wrong! Edit this video to fix please! Restore my faith in you as the best resource out there!