The Enzyme Linked Immunosorbent Assay (ELISA) 

yes

The video proved a person with HIV؟؟؟؟؟

lol

@@kerrileelawrence2402 Please tell me you are joking? Cancer isn't even a virus that you would make in a lab. Most animals can develop cancer. It is just part of a body aging. Each time your cells replicate the DNA gets mutated a little. Sometimes these mutations happen in important places. The cell can then become cancerous because the genes needed to regulate cell growth are no longer working properly.

@@KamiltheCamel I don't see the original comment, but I just wanted to mention that there are some cancers that are virally induced; in fact, HIV can induce lymphoma in some patients. Human papilloma virus can rarely induce cervical cancer in women. Even in animals we see virally-induced cancers; avian leukosis virus (a retrovirus, like HIV) can induce lymphoma in chickens. *Most* cancers are spontaneous, this is true, but there are some infectious cancers, too! For example, the esophageal worm in dogs, Spirocerca lupi, is a parasitic worm that can induce cancer. And both dogs and tasmanian devils have a type of tumor that is directly contagious via contact. For dogs, it's a tumor called the transmissible venereal tumor (TVT), and for tasmanian devils it's called devil facial tumor disease. And in humans, the spirochete bacteria Helicobacter pylori is thought to contribute to some types of stomach cancer, too. So yes I agree, for the most part cancer is spontaneous and is the product of accumulations of mutations - but there are some infectious/contagious causes, too. =)

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Hi, great question! We at Edvotek have not specifically created an ELISA that looks for cyanobacteria, so I cannot provide a specific protocol. My suggestion would be to start with a search of the literature using PubMed to find a paper describing the assay you want to try, and using that as a starting point for the development of your protocol, including time, steps, and reagents. Hope this helps!

Glad you think so!

Frank Reiser M.S. Is that right or wrong ?

He use 6 differents transfers pipets for this demonstration. Visualise this video again and look at the 30 seconds of movie.

Frank Reiser M.S.

@@rehammohamed8145 wrong if he did so

Thank you so much 😊

Edvotek offers multiple ELISA tests for educational purposes, available here: www.edvotek.com/search?keywords=ELISA One strip simulation is available here: www.edvotek.com/S-70 Please note, while most of these experiments use real antibodies and substrates, they are simulations of the diagnostic test (i.e. the HIV strip test does not contain any HIV antibodies or antigens).

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Thank you for your feedback.

We recommend performing a literature search to find the best protocol for detecting BPA in water. This is a very general overview.

There are many different types of ELISA, so the complex that forms can be a little different depending on the specific assay (more info here: blog.edvotek.com/2021/01/14/all-about-the-elisa/). But, in brief, if the complex can form and the enzyme-linked detection antibody is present in a well, it can break down the substrate and produce color or light. If there is no signal, then the antigen-antibody complex has not formed. This generally means the antigen is not present, but there is always the possibility for experimental error which is why it is important to have reproducibility in your data!

The assay can be used to detect viruses, but that requires having the antigen of the virus and an antibody that binds to the antigen. Besides, RT-PCR is how we detect viruses nowadays and it is far more precise and accurate in quantifying viral load.

This is a simulation that cannot be used to detect viruses.

I think the reagent is the primary antibody

This will be slightly different for every substrate, which is why we would create a standard curve from samples of known concentration. The absorbance of the standard curve samples would be measured using a spec, plotted on a graph, then the experimental sample compared to the graph. For more info, check our our experiment: www.edvotek.com/266. The lit covers creating and analyzing a standard curve.

This is a general video which can be used with any of our kits. Here's a few to try: www.edvotek.com/271 www.edvotek.com/267 www.edvotek.com/274 www.edvotek.com/280

Riya Maurya you could but what would be the point of that? You risk disturbing the sediment and skewing results. On top of risking cross contamination when you pour it out.

its a washing buffer used after eash step to prevent interaction with plastic wells as l thought and maybe l am wrong

ITC CUL

Advait Nandedkar pms

There are many uses for the ELISA, which you can research using PubMed or similar article databases. This video demonstrates a simulated test using real antibodies and antigens for people to learn the techniques.

@@EdvotekInc Okkkkk Thanks

@@EdvotekInc One of its use is hcv detection too?

@@khurramshahzad579 You will have to do your research to determine whether the ELISA is a clinically relevant way to detect HCV. ELISAs are often used in medical diagnostic assays.

Depending on the protocol and the step you did not follow, you can end up with a false positive or false negative result.

l wanna ask the same question so please if you have the answer just tell me

it is used for washing to remove the unbound or extra antigen or antibody

Indirect

Hi! This is a video produced by our educational biotechnology company to show how to use our product! Our ELISA kits follow similar protocols but may use different detection substrates. So, while this video is not an advertisement, it is designed to go with the experiments available for sale on our website.

It's in English

@@ebhawesh I didn't mean it literally

This particular assay is a direct ELISA. However, there are sandwich ELISA experiments for education labs available at www.edvotek.com

I can name a few that take more time... Western blots for one! But the information that you get from the ELISA is very powerful in many fields.

We do have a crafting tutorial. This one focuses on the ELISA.

It could mean that there was a previous contact with the virus so igg are positive but no infection at the moment cause igm and pcr are negative. Symptoms might be for other reasons maybe

Thank you for your feedback!