Vertical vs Horizontal Laminar Flow Hood 

At this very moment in the design phase you have been instrumental in helping me make the decision to 'Go Horizontal'. LOL Thanks Gary, great content.

I have grown to really appreciate your channel. Appreciate the work you are doing, Gary.

This is really good info to know when starting out, to help determine the best one for whatever the goals are, thanks

Thanks for clearing that up!

Bro thank you!

Tru clean rooms pull through the floor. Get your intake air from the grated counter and send it through your filters. You’ll capture all contaminants and preserve your filter cause it’s getting most of supply already clean

I made a horizontal 12 x 24 FH ;but to overcome the potential for airborne particulates from the sides/above becoming contamination vectors I extended the lower sides and bottom to create a work area (lined with 1/4 inch plastic like black and smooth material I had), plus I installed a double hinged half inch thick plexi door that covers the ceiling in the work area and the second half when not in use drops down to close the work area off. I'm actually going one step further and installing a UV-C light to scrub the air in the plenum (where there's zero chance of exposure to anything living). I like what you said about the still air box aspect when needing to clean up contaminated culture (something I'm actually sickly obsessed with lol) and am going to cut out coverable arm holes now. One other often overlooked downside of horizontal FH is also that the flat working surface usually sits below the lowest part of the flow coverage, easily fixed by using a rack ;but in my testing proven to be a contamination vector.

Great videos

A separate glove box for isolating from unknown contamination will help keep your primary clean room healthy.

Hi Gary. What is the difference between the Flow model and the LF model from Air Science? Thanks.

Hey Gary, thanks for this video! I’ve been wondering about this watching your other videos. With the vertical flow, is it even more pertinent to not cross your hands over open containers?

Hi Gary, would you mind just describing some of the most important techniques/tips for success that you've found when working with vertical laminar flow?

What model hood is being used in this video?

You guys hiring? I would love to work in a lab like this growing mushrooms. Air Science is great. I was looking at those before I got mine. I have a 2'x4' Envirco Mac10 LEAC I just got and I love it. I use it horizontal at the moment but after hearing what you said about vertical it has me wanting to go vert. I'm going to try to find a 2'x4' housing to mount my filter on.

Hey Gary - just wanted to ask: I have a small vertical hood, should I run it on full or at like 150 pascal? I heard one shouldn't necessarily run them on full?

I am planning to buy one. But not sure whether I should get a vertical or a horizontal. I do microbiology work. There is lots of space in my lab. Which one should I go for?

why do you have pressure canners if you use a autoclave? trying to learn

Hey Gary, are you able to share what model flow hood you have from Air Science?

do you recommend vertical or horizontal when inoculating sawdust grow bags with mycelium grain spawns?

Which type of laminar air flow is more effective in microbiology department in a pharmaceutical company? vertical or horizontal?

Good stuff mang! What model did you buy? Why not get a BSC (Biological Safety Cabinet)?

I use similar. Love it. Whole space is sterile.

Do you think a fume hood can be turned into a flow hood? 🤔

Meant to say "Go Vertical".

"the air is flowing from the top down". Actually, it's not. If there were no table underneath, the air would be flowing in a laminar pattern from top to bottom. With the table, the flow is turbulent as it is being directed forward, the only direction it has to go. This means you have a high pressure gradient with the highest in the top-back, second highest top-front, third highest bottom-back, and lowest pressure zone in the bottom-front. I'd almost predict that there would be a turbulent eddy at the bottom back where much less air is actually being exchanged. I wouldn't be surprised if you put smoke there if it would dwell and swirl for some time. I'm not saying it's not effective though, however less reliable than true laminar flow IMO, especially if you are moving quickly.

Why aren’t you using a bio safety cabinet for the more sensitive work?

You are not wrong that with a horizontal hood the jars closest to the hood itself are in a more sterile environment. You are, however, incorrect that the ones towards the front are in just as sterile air as the ones in the back. The problem with vertical is the flow of the air will pull air from the surrounding area just outside of the hood. Any particles that might be pulled into the flow of the vertical hood, due to that, could get caught in the current and be blown directly on top of your jars, or whatever else you're working on. With a horizontal hood, any particles pulled into the flow will be blown out and away from your work area right away.

I need help once I put my grain into the pellets and seal bags,, growth seems to stop . I spend hrs watching your vids ... Please advise